Monodispersed Mesoporous Silica Nanoparticles with Very Large Pores for Enhanced Adsorption and Release of DNA
Monodispersed Mesoporous Silica Nanoparticles with Very Large Pores for Enhanced Adsorption and Release of DNA
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DOI：
10.1021/jp807956r
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发表时间：
2009-02-12
影响因子：
3.3
通讯作者：
Dong, Lin
中科院分区：
文献类型：
--
作者：
Gao, Fei;Botella, Pablo;Dong, Lin
在双表面活性剂体系的存在下，通过低温（10 ℃）合成方法制备具有受控直径（类似于70-300 nm）和具有20 nm均匀孔的二氧化硅纳米颗粒。虽然三嵌段共聚物（普朗尼克F127）作为超分子模板和coassembles与水解二氧化硅物种开发一个部分有序的面心立方对称的中间相，具有高表面活性的氟碳表面活性剂（FC-4）通过S+X-I+相互作用包围的二氧化硅颗粒，从而限制它们的生长。该材料的最终纹理特性是通过随后的水热处理来实现的，以产生迄今为止报道的具有最大孔入口尺寸（17 nm）和腔直径（20 nm）的高孔体积介孔二氧化硅纳米颗粒。在用氨丙基进行表面改性后，纳米颗粒能够将萤火虫荧光素酶质粒DNA（pGL 3-Control，5256 pb）的分子包封在孔内，导致具有高达0.07 μ g DNA m（-2）的稳定缀合物，这是用二氧化硅基材料实现的最高含量。此外，当与介观结构的纳米颗粒缀合时，质粒DNA被保护免于酶促降解。
Silica nanoparticles with controlled diameter (similar to 70-300 nm) and with uniform pores of 20 nm are prepared by a low temperature (10 degrees C) synthetic method in the presence of a dual surfactant system. While a triblock copolymer (Pluronic F127) acts as supramolecular template and coassembles with hydrolyzed silica species to develop a partially ordered mesophase with face-centered cubic symmetry, a fluorocarbon surfactant with high surface activity (FC-4) surrounds the silica particles through S+X-I+ interactions, thereby limiting their growth. The final textural properties of this material are achieved by means of a subsequent hydrothermal treatment to yield high pore volume mesoporous silica nanoparticles with the largest pore entrance size (17 nm) and cavity diameter (20 nm) reported up to now. After surface modification with aminopropyl groups, the nanoparticles are able to encapsulate inside the pores molecules of the firefly luciferase plasmid DNA (pGL3-Control, 5256 pb), leading to stable conjugates with up to 0.07 mu g DNA m(-2), which is the highest content achieved with silica-based materials. Furthermore, plasmid DNA becomes protected from enzymatic degradation when conjugated with the mesostructured nanoparticles.