STAPHYLOCOCCAL ENTEROTOXIN-A IS ENCODED BY PHAGE
STAPHYLOCOCCAL ENTEROTOXIN-A IS ENCODED BY PHAGE
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DOI：
10.1126/science.3160112
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
MEKALANOS, JJ
中科院分区：
文献类型：
--
作者：
BETLEY, MJ;MEKALANOS, JJ
葡萄球菌肠毒素A （entA）基因在2个野生型菌株中由相关温度噬菌体携带。对转化entA的噬菌体PS42-D及其细菌宿主的DNA进行杂交分析表明，该噬菌体通过循环和互惠交叉（the Campbell模型）整合到细菌染色体中，并且entA基因位于噬菌体附着位点附近。8株不产生肠毒素A的葡萄球菌中的3株和7株产生肠毒素A的野生型（EntA+）葡萄球菌的DNA与转化肠毒素的噬菌体PS42-D DNA具有广泛的同源性，尽管存在高度的限制性片段长度多态性。至少1株EntA+菌株在诱导后未产生可检测到的活菌体。金黄色葡萄球菌噬菌体的多态家族（其中一些可能有缺陷）可以明显携带entA基因。
The gene for staphylococcal enterotoxin A (entA), in 2 wild-type strains, is carried by related temperature bacteriophages. Hybridization analysis of DNA from entA-converting phage PS42-D and its bacterial host suggests that this phage integrates into the bacterial chromosome by circularization and reciprocal crossover (The Campbell model) and that the entA gene is located near the phage attachment site. DNA from 3 of 8 staphylococcal strain that did not produce enterotoxin A and 7 wild-type enterotoxin A-producing (EntA+) strains had extensive homology to the entA-converting phage PS42-D DNA, although there was a high degree of restriction-fragment length polymorphisms. At least 1 EntA+ strain did not produce detectable viable phage after induction. A polymorphic family of Staphylococcus aureus phages (some of which may be defective) can apparently carry the entA gene.