Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis.
Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis.
复制标题
估计棘球蚴病高发地区家犬中棘球绦虫的患病率
DOI：
10.1186/s40249-018-0458-8
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发表时间：
2018-08-09
影响因子：
8.1
通讯作者：
Yang YR
中科院分区：
文献类型：
--
作者：
Liu CN;Xu YY;Cadavid-Restrepo AM;Lou ZZ;Yan HB;Li L;Fu BQ;Gray DJ;Clements AA;Barnes TS;Williams GM;Jia WZ;McManus DP;Yang YR
囊型包虫病(CE)和泡型包虫病(AE)在宁夏回族自治区中国西吉县是一种高度流行的疾病，几十年来在该县开展了以吡喹酮驱狗为基础的防治工作。为了解细粒棘球绦虫和多房棘球绦虫在家犬中的流行情况，监测包虫病传播动态。方法利用地理信息系统(GIS)景观格局选择研究村为包虫病传播“热点”，并结合医院病历确定包虫病和包虫病的危险区域。2012年，对来自25个选定村庄的750只家犬进行了调查，包括铜抽样和主人问卷。应用多重聚合酶链式反应技术对犬细粒棘球绦虫和多房棘球绦虫进行了特异性诊断。使用IBM SPSS统计软件进行数据分析，以比较两种棘球绦虫的患病率。犬在西吉四个地理区域之间经χ-2检验。结果西吉县西北部地区的驱虫率最高，为84.0%，且西吉县4个地理区域的犬只间驱虫率差异有统计学意义(P <； )。西吉市西北部多房棘球绦虫感染率最高(19.7%，59/300)，西吉市西南部细粒棘球绦虫感染率最高(18.1%，38/210)。西吉市西北部、西南部、东北部和东南部的细粒棘球绦虫感染率无显著差异(P >； )，但4个地区感染细粒棘球绦虫的犬之间有显著差异(P <； )。结论西吉县犬细粒棘球绦虫和多房棘球绦虫均有较高的感染率。西吉县地理危险因素对多房棘球绦虫传播的影响大于细粒棘球绦虫。狗有可能在当地西吉社区内维持这两种棘球绦虫的传播，而目前对狗的吡喹酮剂量似乎在这一地区无效或执行不力。
BackgroundCystic echinococcosis (CE) and alveolar echinococcosis (AE) are highly endemic in Xiji County of Ningxia Hui Autonomous Region (NHAR) in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding decades. This study is to determine the current prevalence of Echinococcus granulosus and E. multilocularis in domestic dogs and monitor the echinococcosis transmission dynamics.MethodsStudy villages were selected using landscape patterns (Geographic Information System, GIS) for Echinococcus transmission “hot spots”, combined with hospital records identifying risk areas for AE and CE. A survey of 750 domestic dogs, including copro-sampling and owner questionnaires, from 25 selected villages, was undertaken in 2012. A copro-multiplex PCR assay was used for the specific diagnosis of E. granulosus and E. multilocularis in the dogs. Data analysis, using IBM SPSS Statistics, was undertaken, to compare the prevalence of the two Echinococcus spp. in dogs between four geographical areas of Xiji by the χ2 test. Univariate analysis of the combinations of outcomes from the questionnaire and copro-PCR assay data was carried out to determine the significant risk factors for dog infection.ResultsThe highest de-worming rate of 84.0% was found in the northwest area of Xiji County, and significant differences (P < 0.05) in the de-worming rates among dogs from the four geographical areas of Xiji were detected. The highest prevalence (19.7%, 59/300) of E. multilocularis occurred in northwest Xiji, though the highest prevalence (18.1%, 38/210) of E. granulosus occurred in southwest Xiji. There was no significant difference (P > 0.05) in the prevalence of E. granulosus in dogs from the northwest, southwest, northeast, and southeast of Xiji, but there were significant differences (P < 0.05) between dogs infected with E. multilocularis from the four areas. None of the other independent variables was statistically significant.ConclusionsThe results from this study indicate a high prevalence of both E. granulosus and E. muiltilocularis in dogs in Xiji County, NHAR. Transmission of E. multilocularis was more impacted by geographical risk-factors in Xiji County than that of E. granulosus. Dogs have the potential to maintain the transmission of both species of Echinococcus within local Xiji communities, and the current praziquantel dosing of dogs appears to be ineffective or poorly implemented in this area.