Biodegradable fumarate-based polyHIPEs as tissue engineering scaffolds
Biodegradable fumarate-based polyHIPEs as tissue engineering scaffolds
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DOI：
10.1021/bm7007235
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发表时间：
2007-12-01
影响因子：
6.2
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Christenson, Elizabeth M.;Soofi, Wafa;Mikos, Antonios G.
聚HIPE显示出巨大的前景作为组织工程支架由于巨大的控制孔径和互连性提供了这种技术。通过聚合含有大分子单体聚富马酸丙二醇酯（PPF）和交联剂富马酸丙二醇酯二丙烯酸酯（PFDA）的高内相乳液（HIPE）的连续相，制备高度多孔、完全生物降解的支架。使用甲苯作为稀释剂以降低有机相的粘度，从而能够形成HIPE。通过改变稀释剂浓度（40- 60wt%）、交联剂浓度（25- 75wt%）和大分子单体分子量（M-n = 800-1000 g/mol）来产生一系列不同孔径和形态的聚HIPE支架。尽管某些配方产生大孔整料（孔径> 500 μ m），但所研究的大多数聚HIPE是刚性的、微孔整料，平均孔径在10-300 μ m范围内。重量分析证实微孔整料的孔隙率为80-89%，大多数支架高于84%。这些研究表明，乳液模板可用于产生刚性的，可生物降解的支架，具有高度互连的孔，适用于组织工程支架。
PolyHIPEs show great promise as tissue engineering scaffolds due to the tremendous control of pore size and interconnectivity afforded by this technique. Highly porous, fully biodegradable scaffolds were prepared by polymerization of the continuous phase of high internal phase emulsions (HIPEs) containing the macromer poly(propylene fumarate) (PPF) and the cross-linker propylene fumarate diacrylate (PFDA). Toluene was used as a diluent to reduce the viscosity of the organic phase to enable HIPE formation. A range of polyHIPE scaffolds of different pore sizes and morphologies were generated by varying the diluent concentration (40-60 wt %), cross-linker concentration (25-75 wt %), and macromer molecular weight (M-n = 800-1000 g/mol). Although some formulations resulted in macroporous monoliths (pore diameter > 500 mu m), the majority of the polyHIPEs studied were rigid, microporous monoliths with average pore diameters in the range 10-300 mu m. Gravimetric analysis confirmed the porosity of the microporous monoliths as 80-89% with most scaffolds above 84%. These studies demonstrate that emulsion templating can be used to generate rigid, biodegradable scaffolds with highly interconnected pores suitable for tissue engineering scaffolds.