Antioxidant activity of the microalga Spirulina maxima
Antioxidant activity of the microalga Spirulina maxima
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DOI：
10.1590/s0100-879x1998000800007
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发表时间：
1998-08-01
影响因子：
2.3
通讯作者：
Mancini, J
中科院分区：
文献类型：
--
作者：
Miranda, MS;Cintra, RG;Mancini, J
螺旋藻是一种富含蛋白质和其他必需营养素的微藻，被用作食品添加剂。螺旋藻含有酚酸、生育酚和a-胡萝卜素，这些物质具有抗氧化特性。本研究的目的是评价螺旋藻提取物的抗氧化能力。测定了螺旋藻甲醇提取物的体内和体外抗氧化活性。体外抗氧化能力在37℃的脑匀浆液中进行了测试。该系统中提取物的IC50（使氧化减少50%的浓度）为0.18 mg/ml。在连续2周和7周每天给药5mg的动物的血浆和肝脏中评估了体内抗氧化能力。测定37℃下脑匀浆1 h的血浆抗氧化能力，测定对照组和试验组在37℃下孵育2 h后肝脏中氧化化合物的生成，以硫代巴比妥酸反应物（TBARS）计。治疗后，实验组血浆抗氧化能力为71%，对照组为54%。肝脏自发过氧化研究的数据在组间无显著差异。测定了螺旋藻提取物中酚酸、α -生育酚和β -胡萝卜素的含量。结果表明，螺旋藻对体内和体外系统均具有一定的抗氧化保护作用。
Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients, Spirulina contains phenolic acids, tocopherols and a-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37 degrees C. The IC50 (concentration which causes a 50% reduction of oxidation) of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and 7 weeks. Plasma antioxidant capacity was measured in brain homogenate incubated for 1 h at 37 degrees C, The production of oxidized compounds in liver after 2 h of incubation at 37 degrees C was measured in terms of thiobarbituric acid reactant substances (TBARS) in control and experimental groups. Upon treatment, the antioxidant capacity of plasma was 71% for the experimental group and 54% for the control group. Data from liver spontaneous peroxidation studies were not significantly different between groups. The amounts of phenolic acids, alpha-tocopherol and beta-carotene were determined in Spirulina extracts. The results obtained indicate that Spirulina provides some antioxidant protection for both in vitro and in vivo systems.