The apple U-box E3 ubiquitin ligase MdPUB29 contributes to activate plant immune response to the fungal pathogen Botryosphaeria dothidea
The apple U-box E3 ubiquitin ligase MdPUB29 contributes to activate plant immune response to the fungal pathogen Botryosphaeria dothidea
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苹果 U-box E3 泛素连接酶 MdPUB29 有助于激活植物对真菌病原体 Botryosphaeria dothidea 的免疫反应
DOI：
10.1007/s00425-018-03069-z
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发表时间：
2019-04-01
期刊：
影响因子：
4.3
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
Han, Peng-Liang;Dong, Yuan-Hua;Hao, Yu-Jin
MdPUB29可能通过直接调控水杨酸（salicylic acid， SA）含量以及SA合成相关和信号相关基因转录，对真菌病原菌Botryosphaeria dothidea的防御反应起到正向调节作用。在植物中，含有U-box结构域的泛素E3连接酶（pub, Plant U-box E3 ubiquitin ligase）已被确定为基本细胞过程的关键调节因子，如细胞生长、发育和凋亡，以及生物和非生物胁迫反应。然而，酒馆在苹果环腐病中的作用尚不清楚。本研究从苹果品种“Royal Gala”中分离出U-box E3连接酶MdPUB29，并对其在植物病原菌防御中的作用进行了研究。qRT-PCR结果显示，苹果果实中MdPUB29的表达受到显著诱导。苹果愈伤组织中MdPUB29基因的过表达增强了苹果对黑僵菌的抗性。相比之下，在苹果愈伤组织中沉默MdPUB29导致抗性降低。与野生型（Col）对照相比，MdPUB29在拟南芥中的异位表达也表现出对dothidea感染的抗性增强。此外，与野生型相比，植物抗病能力的增强与水杨酸（SA）含量的增加以及SA合成相关和信号相关基因转录的增加有关。我们阐明了MdPUB29可能通过调控SA通路提高植物对dothidea病原菌防御的机制。
MdPUB29 is a positive regulator of the defense response to the fungal pathogen Botryosphaeria dothidea possibly by directly regulating the salicylic acid (SA) content as well as SA synthesis-related and signaling-related gene transcription. In plants, ubiquitin E3 ligases containing a U-box domain (PUBs, Plant U-box E3 ubiquitin ligase) have been identified as key regulators of fundamental cellular processes, such as cellular growth, development, and apoptosis, as well as biotic and abiotic stress responses. However, the function of PUBs in apple ring rot remains elusive. Here, we isolated the U-box E3 ligase MdPUB29 from the apple cultivar ‘Royal Gala’ and characterized its function in plant pathogen defense against Botryosphaeria dothidea. qRT-PCR showed that the expression of MdPUB29 was significantly induced in apple fruits after B. dothidea infection. Overexpression of the MdPUB29 gene in apple calli increased the resistance to B. dothidea infection. In contrast, silencing MdPUB29 in apple calli resulted in reduced resistance. Ectopic expression of MdPUB29 in Arabidopsis also exhibited enhanced resistance to B. dothidea infection compared to that of the wild-type (Col) control. In addition, it was found that the increase of plant pathogen defense was correlated with the increased salicylic acid (SA) content, as well as SA synthesis-related and signaling-related gene transcription in comparison to the wild type. We elucidated the mechanism by which MdPUB29 elevates plant pathogen defense against B. dothidea possibly by regulating the SA pathway.