The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
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DOI：
10.1007/s11103-010-9639-y
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发表时间：
2010-07-01
影响因子：
5.1
通讯作者：
Desnos, Thierry
中科院分区：
文献类型：
--
作者：
Creff, Audrey;Sormani, Rodnay;Desnos, Thierry
许多真核生物的基因组都经历了古老的全基因组复制（WGD），然后是大量的基因丢失。这些消除不是随机的，因为一些基因家族优先保留为重复。基因平衡假说认为，剂量减少的基因会使它们相互作用的伴侣或复合体失衡，导致适应性下降。在拟南芥中，胞质核糖体蛋白（RP）由至少两个成员的基因家族编码。我们的研究集中在两个RPS 6基因上，试图理解为什么它们被保留为重复基因。我们证明了RPS 6功能对植物至关重要。我们还表明，降低RPS 6积累水平（在敲除rps 6a或rps 6 b单突变体中，或在双杂合RPS 6A/rps 6a，RPS 6 B/rps 6 b中），赋予缓慢生长表型（单倍缺陷）。重要的是，我们证明了两个RPS 6基因的功能是冗余和可互换的。最后，像大多数其他描述的拟南芥rp突变体，我们观察到，降低RPS 6水平略有改变背腹叶图案。我们的研究结果支持的想法，拟南芥RPS 6基因重复进化保留，以保持必要的表达水平，以维持细胞的翻译需求，与基因平衡假说一致。
Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interacting partners or complex, resulting in decreased fitness. In Arabidopsis, the cytoplasmic ribosomal proteins (RP) are encoded by gene families with at least two members. We have focused our study on the two RPS6 genes in an attempt to understand why they have been retained as duplicates. We demonstrate that RPS6 function is vital for the plant. We also show that reducing the level of RPS6 accumulation (in the knock-out rps6a or rps6b single mutants, or in the double heterozygous RPS6A/rps6a,RPS6B/rps6b), confers a slow growth phenotype (haplodeficiency). Importantly, we demonstrate that the functions of two RPS6 genes are redundant and interchangeable. Finally, like in most other described Arabidopsis rp mutants, we observed that a reduced RPS6 level slightly alters the dorsoventral leaf patterning. Our results support the idea that the Arabidopsis RPS6 gene duplicates were evolutionarily retained in order to maintain an expression level necessary to sustain the translational demand of the cell, in agreement with the gene balance hypothesis.