Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates
Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates
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DOI：
10.1128/jcm.42.8.3869-3872.2004
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发表时间：
2004-08-01
影响因子：
9.4
通讯作者：
Berdal, KG
中科院分区：
文献类型：
--
作者：
Lovseth, A;Loncarevic, S;Berdal, KG
一种改良的多重PCR方法，用于检测九种金黄色葡萄球菌肠毒素基因(SEA、Seb、Sec、Sed、See、seg、Seh、Sei和Sej)和一种形式的免疫反应性中毒性休克综合征毒素(S.R.星期一和G.A.Bohach，J.Clin)。微生物。37：3411-3414,1999)已经被开发。改进后的聚合酶链式反应方法具有较好的鲁棒性，结果可靠。
A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.