Progressively restricted expression of a new homeobox-containing gene during Xenopus laevis embryogenesis.
Progressively restricted expression of a new homeobox-containing gene during Xenopus laevis embryogenesis.
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发表时间：
1991-04
期刊：
影响因子：
4.6
通讯作者：
M. Su;H. Suzuki;M. Solursh;F. Ramirez
中科院分区：
文献类型：
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作者：
M. Su;H. Suzuki;M. Solursh;F. Ramirez
我们已经分离到编码一种新的非洲爪哇同源结构域蛋白的cDNA，该蛋白与小鼠HOX-7.1和果蝇肌肉片段Homebox(MSH)同源。Northern杂交和RNAase保护实验证明，青蛙基因的转录本，称为Xhox-7.1，大约在原肠发育的开始出现。在迅速增加之后，mRNA水平在神经胚期和尾芽中期之间停滞不前，然后稳步下降。原位杂交将Xhox-7.1定位于原肠胚期胚胎背侧中胚层外套膜。对尾芽期胚胎逐渐向前横切面的杂交模式进行比较，将信号定位到背侧神经管和神经脊，定位于侧板中胚层的特定区域，并定位于心源性区域。到蝌蚪阶段，Xhox-7.1信息只出现在中枢神经系统的特定位置，如背侧后脑。因此，在胚胎发育期间，Xhox-7.1的表达水平随着转录本变得更加本地化而降低。最后，证据表明，一个独特的MSH样转录本(暂时命名为Xhox-7.1‘)在原肠胚早期也开始积累。
We have isolated cDNAs encoding a novel Xenopus homeodomain-containing protein homologous to the mouse Hox-7.1 and the Drosophila muscle segment homebox (msh). Northern blot and RNAase protection experiments established that transcripts of the frog gene, termed Xhox-7.1, first appear at about the beginning of gastrulation. After a rapid increase, mRNA levels plateau between the neurula and middle-tailbud stages, and decrease steadily thereafter. In situ hybridization localized the Xhox-7.1 message to the dorsal mesodermal mantle of gastrula stage embryos. Comparison of the hybridization patterns of progressively more anterior cross-section of tailbud stage embryos localized the signal to the dorsal neural tube and neural crest, to specific regions of the lateral plate mesoderm, and to the cardiogenic region. By the tadpole stage, the Xhox-7.1 message appears only at specific sites in the central nervous system, such as in the dorsal hindbrain. Thus, during embryonic development levels of Xhox-7.1 expression decrease as the transcript becomes more progressively localized. Finally, evidence is presented of a distinct msh-like transcript (provisionally termed Xhox-7.1') which begins to accumulate at early-gastrula stage, as well.