Particle and nanoparticle interactions with fibrinogen: the importance of aggregation in nanotoxicology
Particle and nanoparticle interactions with fibrinogen: the importance of aggregation in nanotoxicology
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DOI：
10.3109/17435390.2010.489724
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发表时间：
2011-03-01
期刊：
影响因子：
5
通讯作者：
Kendall, Kevin
中科院分区：
文献类型：
--
作者：
Kendall, Michaela;Ding, Ping;Kendall, Kevin
摄入、吸入或注射的颗粒会与含有聚合物（例如蛋白质纤维蛋白原）的生物液体接触。我们研究了充分表征的亚微米颗粒或纳米颗粒 (NP) 与人纤维蛋白原之间的相互作用。对悬浮在纤维蛋白原溶液中的不同尺寸和功能化的聚苯乙烯、炭黑和二氧化硅纳米粒子进行了体外聚集和 zeta 电位测量。纤维蛋白原存在时，颗粒尺寸、表面电荷和聚集行为发生显着变化。观察到聚合物（蛋白质）桥接和桥絮凝。我们得出结论：（1）纤维蛋白原溶液中的纳米颗粒聚集率取决于颗粒表面类型； (2)胺功能化颗粒在纤维蛋白原中聚集更慢； (3) 颗粒形态强烈影响蛋白质附着的生物可用表面，但这与复杂颗粒的颗粒表面积（计算或测量）没有很好的相关性。因此，颗粒和纳米颗粒与促凝聚合物的相互作用可能决定纳米颗粒表面剂量呈现给细胞/器官以及随后体内和离体的细胞效应。
Ingested, inhaled or injected particles come into contact with biological fluids containing polymers, such as the protein fibrinogen. We studied interactions between well-characterized submicron particles or nanoparticles (NPs) and human fibrinogen. In vitro aggregation and zeta potential measurements of different sized and functionalized polystyrene, carbon black and silica NPs suspended in fibrinogen solutions were made. Particle size, surface charge and aggregation behaviour significantly changed in the presence of fibrinogen. Polymer (protein) bridging and bridge flocculation was observed. We concluded: (1) NP aggregation rate in a fibrinogen solution depended on particle surface type; (2) amine-functionalized particles aggregated more slowly in fibrinogen; and (3) particle morphology strongly influenced biologically available surface for protein attachment, but this did not correlate well with particle surface area for complex particles (calculated or measured). Interaction of particles and NPs with pro-coagulant polymers may therefore dictate the NP surface dose presentation to cells/organs and subsequent cellular effects, in and ex vivo.