Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori.
Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori.
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幽门螺杆菌八异戊二烯焦磷酸合酶的表达、结晶和初步晶体学研究。
DOI：
10.1107/s1744309110051511
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Defeng Li
中科院分区：
文献类型：
--
作者：
Jinyong Zhang;Xiaoli Zhang;X. Mao;Q. Zou;Defeng Li
八异戊二烯焦磷酸合酶（OPPs）参与泛醌和甲基萘醌侧链的合成，并催化焦磷酸法呢酯与焦磷酸异戊烯酯的连续缩合反应以生成焦磷酸聚异戊二烯酯和焦磷酸。为了研究OPPs在泛醌和甲基萘醌代谢中的作用以及这些酶的作用机制，本研究对幽门螺杆菌OPPs的结构与功能关系进行了分析。OPPs的基因被克隆，蛋白质被表达、纯化和结晶，并且衍射数据集被收集到2.00 nm分辨率。 晶体属P4（1）2（1）2或P4（3）2（1）2空间群，晶胞参数a = B = 109.33，c = 103.41。
Octaprenyl pyrophosphate synthase (OPPs) is involved in the synthesis of the side chains of ubiquinone and menaquinone and catalyzes consecutive condensation reactions of farnesyl pyrophosphate with isopentenyl pyrophosphate to generate polyprenyl pyrophosphate and pyrophosphate. In order to investigate the roles played by OPPs in the metabolism of ubiquinone and menaquinone and the enzymatic mechanisms of these enzymes, analysis of the structure-function relationship of OPPs from Helicobacter pylori was initiated. The gene for OPPs was cloned, the protein was expressed, purified and crystallized and a diffraction data set was collected to 2.00 Å resolution. The crystals belonged to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 109.33, c = 103.41 Å.