Genetic impacts induced by BaP and Pb in Mytilus coruscus: Can RAPD be a validated tool in genotoxicity evaluation both in vivo and in vitro?
Genetic impacts induced by BaP and Pb in Mytilus coruscus: Can RAPD be a validated tool in genotoxicity evaluation both in vivo and in vitro?
复制标题
BaP 和 Pb 在贻贝中引起的遗传影响：RAPD 能否成为体内和体外遗传毒性评估的有效工具？
DOI：
10.1016/j.ecoenv.2018.11.066
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发表时间：
2019-03-01
影响因子：
6.8
通讯作者：
Di, Yanan
中科院分区：
文献类型：
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作者：
Qu, Mengjie;Ding, Jiawei;Di, Yanan
苯并(A)芘(BaP)和铅(Pb2+)是海洋中大量排放的常见污染物，对海洋生物造成了严重的危害。虽然贻贝是最重要和研究最多的生物模型之一，但关于两种完全不同的化学物质混合引起的贻贝基因组变化的研究仍然很少。本研究采用BaP(53.74+/-19.79g/L)、铅(2.58+/-0.11 mg/L)和BaP(53.74+/-19.79g/L)及其混合物对本地贻贝进行体内暴露6d。用彗星试验、微核试验(MNI)和随机扩增多态性DNA(RAPD)分析评价其遗传毒性损伤。尽管在暴露后调查了短暂的基因组损伤，但显着增加，并通过各种检测方法显示出一致性。通过MNI试验，只有在联合暴露3天后才发现加性遗传毒性，这表明BaP和Pb可能在代谢和/或与基因组相互作用的过程中发挥着替代的生物靶标作用。在体内和体外进一步检测BaP攻击后的基因稳定性和恢复能力。随机扩增结果显示，BaP的遗传毒性与时间特异性变化一致。在本研究中发现，在体内和体外暴露的情况下，基因组的不稳定性都会恢复。据我们所知，这是首次利用多种检测技术对苯并(A)苯、铅及其混合物的遗传毒性进行研究。利用模型污染物和海洋生物来验证随机扩增多态性DNA分析的潜在价值，突出了它可能是遗传毒理学研究的有用工具，特别是在遗传水平上的效应-机制相互作用的研究。
Benzo(a)pyrene (BaP) and lead (Pb) are common pollutants discharged greatly in ocean and causing detrimental impacts on marine organisms. Although mussels are one of the most prominent and frequently studied biological models, the research on their genomic alterations induced by the mixture of two totally different chemicals, is still rare. In present study, local marine mussels Mytilus coruscus were exposed in vivo to BaP (53.74 +/- 19.79 g/ L), Pb (2.58 +/- 0.11 mg/L) and their mixture for 6 days. The genotoxic damages were assessed by comet assay, micronucleus (MNi) test, and random amplified polymorphic DNA (RAPD) analysis. Significantly increased though transitory genomic damage was investigated after the exposure and showed consistency using various detecting methods. Additive genotoxicity was only found after 3 days combined exposure by means of MNi test, suggesting that BaP and Pb may play with alternative biological targets during metabolism and/or interaction with the genome. The geno-stability and the recovery capability were further detected both in vivo and in vitro after challenged by BaP. RAPD results showed coherence in BaP induced genotoxicity, together with time specific alterations. The genomic instability was found to recover in both in vivo and in vitro exposure scenarios in present study. To our knowledge, this is the first study to focus on the genotoxicitiy induced by BaP, Pb and their mixture by multiple detecting techniques. The attempt to utilize model pollutants and marine organism to validate the potential value of RAPD analysis highlighted that it might be a useful tool in the research of genotoxicology, especially on the effect -mechanism interplay at genetic level.