Genome 10K: A Proposal to Obtain Whole-Genome Sequence for 10 000 Vertebrate Species
Genome 10K: A Proposal to Obtain Whole-Genome Sequence for 10 000 Vertebrate Species
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DOI：
10.1093/jhered/esp086
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发表时间：
2009-11-01
影响因子：
3.1
通讯作者：
Turner, Steve
中科院分区：
文献类型：
--
作者：
Haussler, David;O'Brien, Stephen J.;Turner, Steve
人类基因组计划最近得到了32种哺乳动物和24种非哺乳类脊椎动物的全基因组评估序列的补充，适合于比较基因组分析。在这里，我们预计在成本和测序效率的增加，伴随着改进的注释技术的发展急剧下降，因此，建议在不久的将来创建一个专门指定用于全基因组测序的10 000种脊椎动物物种的组织和DNA标本的集合。为此，我们，基因组10K科学家社区（G10KCOS），将收集和分配约16203个代表性脊椎动物物种的生物标本，这些物种跨越了现存哺乳动物、鸟类、非鸟类爬行动物、两栖动物和鱼类的进化多样性。60 000个物种）。在这项提案中，我们提出了精确的计数，这16 203个物种的标本目前标记和规定的DNA测序的G10KCOS。DNA测序开创了生物科学研究的新时代，使我们能够第一次真正全面地研究脊椎动物的进化，其结果将涉及脊椎动物生物学研究的几乎每一个方面。
The human genome project has been recently complemented by whole-genome assessment sequence of 32 mammals and 24 nonmammalian vertebrate species suitable for comparative genomic analyses. Here we anticipate a precipitous drop in costs and increase in sequencing efficiency, with concomitant development of improved annotation technology and, therefore, propose to create a collection of tissue and DNA specimens for 10 000 vertebrate species specifically designated for whole-genome sequencing in the very near future. For this purpose, we, the Genome 10K Community of Scientists (G10KCOS), will assemble and allocate a biospecimen collection of some 16 203 representative vertebrate species spanning evolutionary diversity across living mammals, birds, nonavian reptiles, amphibians, and fishes (ca. 60 000 living species). In this proposal, we present precise counts for these 16 203 individual species with specimens presently tagged and stipulated for DNA sequencing by the G10KCOS. DNA sequencing has ushered in a new era of investigation in the biological sciences, allowing us to embark for the first time on a truly comprehensive study of vertebrate evolution, the results of which will touch nearly every aspect of vertebrate biological enquiry.