TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth
TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth
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TsNAC1 是非生物胁迫抵抗和生长的关键转录因子
DOI：
10.1104/pp.17.01089
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发表时间：
2018-01-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Juren
中科院分区：
文献类型：
--
作者：
Liu, Can;Wang, Baomei;Zhang, Juren
NAC蛋白是植物特异性转录因子中最大的家族之一，其中许多蛋白参与调控植物的发育和对非生物胁迫的应答。T.盐生芥逆境相关NAC 1（TsNAC 1）是从盐生芥中克隆的一个NAC转录因子基因，其过表达可提高盐生芥和盐生芥的非生物胁迫抗性，尤其是耐盐性。盐生植物和拟南芥（Arabidopsis thaliana）并阻碍这些植物的生长。本研究比较了拟南芥TsNAC 1和RD 26与拟南芥TsNAC 1启动子区的转录激活活性。结果表明，TsNAC 1在烟草和酵母中具有较高的转录激活活性。目的基因启动子的靶序列也被鉴定，TsNAC 1被证明靶向离子转运的正调控因子，如T.嗜盐菌H+-PPASE 1和转录因子MYB下胚轴伸长相关和HOMEOBOX 12。此外，TsNAC 1负调节细胞的扩增，抑制光依赖性短下胚轴1和UDP-木糖基转移酶2，并直接控制IRA 14的多拷贝供应的表达。基于这些结果，我们建议TsNAC 1功能作为一个重要的上游调节植物非生物胁迫反应和营养生长。
NAC proteins constitute one of the largest families of plant-specific transcription factors, and a number of these proteins participate in the regulation of plant development and responses to abiotic stress. T. HALOPHILA STRESS RELATED NAC1 (TsNAC1), cloned from the halophyte Thellungiella halophila, is a NAC transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both T. halophila and Arabidopsis (Arabidopsis thaliana) and retard the growth of these plants. In this study, the transcriptional activation activity of TsNAC1 and RD26 from Arabidopsis was compared with the target genes' promoter regions of TsNAC1 from T. halophila, and the results showed that the transcriptional activation activity of TsNAC1 was higher in tobacco (Nicotiana tabacum) and yeast. The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as T. HALOPHILA H+- PPASE1, and the transcription factors MYB HYPOCOTYL ELONGATION-RELATED and HOMEOBOX12. In addition, TsNAC1 negatively regulates the expansion of cells, inhibits LIGHT-DEPENDENT SHORT HYPOCOTYLS1 and UDP-XYLOSYLTRANSFERASE2, and directly controls the expression of MULTICOPY SUPPRESSOR OF IRA14. Based on these results, we propose that TsNAC1 functions as an important upstream regulator of plant abiotic stress responses and vegetative growth.