Putrescine regulates nitric oxide accumulation in Ganoderma lucidum partly by influencing cellular glutamine levels under heat stress
Putrescine regulates nitric oxide accumulation in Ganoderma lucidum partly by influencing cellular glutamine levels under heat stress
复制标题
腐胺部分通过影响热应激下细胞谷氨酰胺水平来调节灵芝中一氧化氮的积累
DOI：
10.1016/j.micres.2020.126521
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发表时间：
2020
影响因子：
6.7
通讯作者：
Ming-wen Zhao
中科院分区：
文献类型：
--
作者：
Jia-le Xia;Chen-Gao Wu;Ang Ren;Yan-ru Hu;Sheng-li Wang;Xiao-fei Han;Liang Shi;Jing Zhu;Ming-wen Zhao
当真菌受到非生物胁迫时，多胺（PAs）水平发生显著变化。在这里，我们发现，多胺腐胺（Put）可以发挥重要作用，减轻热应激（HS）诱导的一氧化氮（NO）的积累。鸟氨酸脱羧酶（ODC）沉默的突变体，是在Put生物合成缺陷表现出显着低于野生型（WT）时，受到HS。加入5 mM外源Put后，突变体内源Put在HS下明显增加。同时，外源Put处理后，ODC沉默突变体的NO含量恢复到野生型水平。而外源Put和一氧化氮清除剂carboxy-PTIO（PTIO是NO的特异性清除剂）的加入使ODC沉默突变体中NO含量升高的现象消失。有趣的是，在ODC沉默的菌株中，谷氨酰胺（Gln）的含量显著增加。在WT中添加外源Put后，谷氨酰胺含量显著降低。高水平谷氨酰胺的出现伴随着硝酸还原酶（NR）活性的降低。进一步的研究表明，在HS条件下，Put可能通过调节NO含量而影响灵芝酸（GAs）的生物合成。我们的工作报告说，Put调节HS诱导的NO积累通过改变细胞Gln水平在丝状fungi.ImportanceIn我们目前的工作，这是HS作为一个无处不在的环境压力，影响重要的药理次级代谢产物（GAs）的含量在G。之后，我们开始探索多种物质之间形成的网络，共同减少HS引起的GAs含量的大量积累。我们首先关注Put，一种增强对多种压力的抵抗力的物质。此外，我们发现，对Put的抑制可以改变NO含量，这已被证明可以减少GAs通过HS的积累。NO含量的变化可能是由于Put水平影响细胞内Gln含量。从来没有报道过。最终，Put相关网络可以减少HS诱导的真菌次级代谢产物的混乱。
When fungi are subjected to abiotic stresses, the polyamines (PAs) level alter significantly. Here, we reveal that the polyamine putrescine (Put) could play an important role in alleviating heat stress(HS)-induced accumulation of nitric oxide (NO).Ornithine decarboxylase (ODC)-silenced mutants that were defective in Put biosynthesis exhibited significantly lower NO levels than the wild type (WT) when subjected to HS. With addition of 5 mM exogenous Put, theODC-silenced mutant endogenous Put obviously increased under HS. At the same time, the contents of NO in theODC-silenced mutants recovered to approximately WT levels after the administration of exogenous Put. However, the elevated NO content in theODC-silenced mutants disappeared when exogenous Put and carboxy-PTIO (PTIO is a specific scavenger of NO) were added. Intriguingly, the content of glutamine (Gln) was significantly increased in theODC-silenced strains. When exogenous Put was added to the WT, the Gln content was significantly decreased. The appearance of a high level of Gln was accompanied by nitrate reductase (NR) activity reduction. Further studies showed that Put influenced ganoderic acids (GAs) biosynthesis by regulating NO content, possibly through NR, under HS. Our work reported that Put regulates HS-induced NO accumulation by changing the cellular Gln level in filamentous fungi.ImportanceIn our present work, it was HS as an ubiquitous environmental stress that affects the important pharmacological secondary metabolite (GAs) content inG. lucidum.Afterwards, we began to explore the network formed between multiple substances to jointly reduce the massive accumulation of GAs content caused by HS. We firstly focused on Put, a substance that enhances resistance to multiple stresses. Further, we discovered an influence on Put could changing the NO content, which has been shown to decrease the accumulation of GAs via HS. Then, we also found the change of NO content may be due to Put level that would affect intracellular Gln content. It has never been reported. And ultimately, it is Put related network that could reduce HS-inducing secondary metabolite mess in fungi.