siRNA-mediated down-regulation of iASPP promotes apoptosis induced by etoposide and daunorubicin in leukemia cells expressing wild-type p53
siRNA-mediated down-regulation of iASPP promotes apoptosis induced by etoposide and daunorubicin in leukemia cells expressing wild-type p53
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siRNA 介导的 iASPP 下调可促进表达野生型 p53 的白血病细胞中依托泊苷和柔红霉素诱导的细胞凋亡。
DOI：
10.1016/j.leukres.2009.02.016
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发表时间：
2009-09-01
影响因子：
2.7
通讯作者：
Wang, Jianxiang
中科院分区：
文献类型：
--
作者：
Liu, Hang;Wang, Min;Wang, Jianxiang
ASPP家族的癌蛋白抑制性成员（iASPP）是肿瘤抑制因子p53的关键抑制剂。本研究采用RNA干扰技术（RNAi）阻断白血病细胞株Nalm6和K562中iASPP的表达，探讨iASPP对白血病细胞的作用。结果表明，内源性iASPP的下调增加了白血病细胞p53依赖的凋亡。因此，iASPP有可能成为白血病治疗的分子靶点。(C)2009年由Elsevier Ltd.出版
Oncoprotein inhibitory member of the ASPP family (iASPP) is a key inhibitor of tumor suppressor p53. Our previous study revealed that the expression of iASPP in acute leukemia (AL) patients was higher than that of normal control which implied that iASPP might play an important role in the pathogenesis and/or disease progression of AL In this study, the iASPP expression was blocked by RNA interference (RNAi) in two leukemic cell lines, Nalm6 and K562, to explore the effects of iASPP on leukemia cells. The results indicated that down-regulation of endogenous iASPP increased p53-dependent apoptosis of leukemia cells. Thus, iASPP could be a molecular target in leukemia therapy. (C) 2009 Published by Elsevier Ltd.