Catalytic properties of Na+-translocating NADH:quinone oxidoreductases from Vibrio harveyi, Klebsiella pneumoniae, and Azotobacter vinelandii
Catalytic properties of Na+-translocating NADH:quinone oxidoreductases from Vibrio harveyi, Klebsiella pneumoniae, and Azotobacter vinelandii
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DOI：
10.1111/j.1574-6968.2007.01015.x
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发表时间：
2008-02-01
影响因子：
2.1
通讯作者：
Bogachev, Alexander V.
中科院分区：
文献类型：
--
作者：
Fadeeva, Maria S.;Nunez, Cinthia;Bogachev, Alexander V.
比较分析了海洋细菌哈氏弧菌、肺炎克雷伯氏菌和土壤微生物棕色固氮菌的钠转运NADH：苯醌氧化还原酶(Na+-NQRs)的催化性能。结果表明，这些酶对钠离子的亲和力有很大的不同。这些酶对抑制剂也具有不同的敏感性。棕色假单胞菌的Na+-NQR对低浓度的2-正庚基-4-羟基喹啉N-氧化物(HQNO)不敏感，而肺炎克雷伯菌的Na+-NQR对Ag+或N-乙基马来酰亚胺具有完全抗性。所有的Na+-NQR型酶都对二苯基碘敏感，这被证明是改变了酶的非共价结合的FAD。
The catalytic properties of sodium-translocating NADH:quinone oxidoreductases (Na+-NQRs) from the marine bacterium Vibrio harveyi, the enterobacterium Klebsiella pneumoniae, and the soil microorganism Azotobacter vinelandii have been comparatively analyzed. It is shown that these enzymes drastically differ in their affinity to sodium ions. The enzymes also possess different sensitivity to inhibitors. Na+-NQR from A. vinelandii is not sensitive to low 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO) concentrations, while Na+-NQR from K. pneumoniae is fully resistant to either Ag+ or N-ethylmaleimide. All the Na+-NQR-type enzymes are sensitive to diphenyliodonium, which is shown to modify the noncovalently bound FAD of the enzyme.