Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues
Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues
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DOI：
10.1016/j.gene.2003.11.017
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发表时间：
2004-03-17
期刊：
影响因子：
3.5
通讯作者：
Novelli, G
中科院分区：
文献类型：
--
作者：
Contino, G;Amati, F;Novelli, G
泛素化因子E4 A基因（UBE 4A）编码U盒型泛素连接酶，最初被描述为E4泛素化因子。UBE 4A是酿酒酵母Ufd 2的哺乳动物同源物。UBE 4A基因定位于人类染色体1q23.3区域，这是参与某些特定癌症如神经母细胞瘤的关键区域。对胎儿和成人组织的北方印迹分析显示，在心脏、骨骼肌和肾脏中最丰富的类似于7.5 kb转录物的单一条带。我们制备了UBE 4A的多克隆抗体，并进行了免疫印迹和免疫组织化学分析。UBE 4A蛋白呈现为约125 kDa的单一条带。UBE 4A存在于骨骼肌、肾脏和肝脏中;外周血白细胞和脾脏中可见一条微弱的条带。我们没有发现UBE 4A在全脑、结肠、肺和心脏中的表达。在细胞水平，UBE 4A主要在皮质神经元和肝脏的细胞核和细胞质中以及肾小管细胞的细胞核中表达。在肝脏中，类似细胞的细胞核似乎未染色或以不同水平染色，表明UBE 4A可能具有细胞周期依赖性表达或在细胞周期控制中的作用。总之，我们的研究结果表明，UBE 4A在不同的组织中表达的模式，似乎是依赖于细胞类型和细胞周期，UBE 4A可能有一个特定的作用，在不同的生化过程以外的泛素化，包括生长或分化。(C)2004 Elsevier B. V.保留所有权利。
The Ubiquitination Factor E4A gene (UBE4A) encodes for a U-box-type ubiquitin ligase, originally described as an E4 ubiquitination factor. UBE4A is a mammalian homolog of Saccharomyces cerevisiae Ufd2. The UBE4A gene has been mapped on the human chromosome region 1 q23.3, a critical region involved in some specific cancers such as neuroblastoma. Northern blots analysis on foetal and adult human tissues revealed a single band of similar to 7.5 kb transcript most abundant in the heart, skeletal muscle and kidney. We generated a polyclonal antibody to UBE4A and performed immunoblot and immunohistochemical analysis. The UBE4A protein appeared as a single band of approximately 125 kDa. UBE4A was present in the skeletal muscle, kidney and liver; a faint band was visible in peripheral blood leukocytes and spleen. We did not reveal expression of UBE4A in whole brain, colon, lung and heart. At the cellular level, UBE4A results predominantly expressed in the nucleus and the cytoplasm of cortical neurons and liver and in the nucleus of tubular kidney cells. In the liver, the nucleus of similar cells appeared to be unstained or stained at different levels suggesting that UBE4A may have a cell cycle dependent expression or a role of in cell cycle control. In conclusion, our results show that UBE4A is expressed in different tissues in a pattem that seems to be dependent from cell type and cell cycle and that UBE4A might have a specific role in different biochemical processes other than ubiquitination, including growth or differentiation. (C) 2004 Elsevier B.V. All rights reserved.