Analysis of the functions of the signal peptidase complex in the midgut of Tribolium castaneum.
Analysis of the functions of the signal peptidase complex in the midgut of Tribolium castaneum.
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赤拟谷盗中肠信号肽酶复合物的功能分析
DOI：
10.1002/arch.21441
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发表时间：
2018
影响因子：
2.2
通讯作者：
Huang Wuren
中科院分区：
文献类型：
--
作者：
Guan Jingmin;Zhang Jie;Yuan Shenglei;Yang Bing;Clark Kevin D;Ling Erjun;Huang Wuren
信号肽酶复合物（SPC）是从细菌到人类的保守蛋白，通常由4 - 5个亚基组成。赤拟谷盗（Tribolium castaneum）中有4个编码SPC蛋白的基因。为了了解它们对昆虫发育的重要性，在早期幼虫和成虫阶段将每个SPC基因的双链RNA注射到红粉甲虫中。敲除所有四个信号肽酶基因对幼虫是致命的。此外，幼虫有困难与旧角质层蜕皮。单独敲除TcSPC 12不影响蛹或成虫的发育。当TcSPC 12、TcSPC 18和TcSPC 25被敲除时，幼虫的血细胞和中肠出现黑化。TcSPC 18基因敲除后，幼虫和成虫中肠细胞发生严重凋亡，成虫中肠失去维持肠腔的能力，表明TcSPC 18基因对中肠细胞增殖和分化的重要性。TcSPC 22或TcSPC 25的敲低也导致中肠中许多凋亡细胞。然而，TcSPC 12似乎对中肠发育不重要。因此，TcSPC 18对维持中肠隐窝的发育是必需的。
Signal peptidase complexes (SPCs) are conserved from bacteria to human beings, and are typically composed of four to five subunits. There are four genes encoding SPC proteins in the red flour beetle,Tribolium castaneum. To understand their importance to insect development, double‐stranded RNA for each SPC gene was injected into red flour beetles at the early larval and adult stages. Knockdown of all four signal peptidase genes was lethal to larvae. Moreover, larvae had difficulty with old cuticle ecdysis. Knockdown ofTcSPC12alone did not affect pupal or adult development. WhenTcSPC12,TcSPC18, andTcSPC25were knocked down in larvae, the melanization of hemocytes and midguts was observed. When knocked down in larvae and adults,TcSPC18induced severe cell apoptosis in midguts, and the adult midgut lost the ability to maintain crypts after knockdown ofTcSPC18, indicating its importance to midgut cell proliferation and differentiation. Knockdown ofTcSPC22orTcSPC25also resulted in many apoptotic cells in the midguts. However,TcSPC12appeared to be unimportant for midgut development. We conclude thatTcSPC18is essential for maintaining the adult midgut crypts.