Dynamic complex formation during the yeast cell cycle
Dynamic complex formation during the yeast cell cycle
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DOI：
10.1126/science.1105103
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发表时间：
2005-02-04
期刊：
影响因子：
56.9
通讯作者：
Bork, P
中科院分区：
文献类型：
--
作者：
de Lichtenberg, U;Jensen, LJ;Bork, P
为了分析酵母细胞周期中蛋白质复合物的动力学，我们整合了有关蛋白质相互作用和基因表达的数据。由此产生的时间依赖性相互作用网络将定期和组成型表达的蛋白质放置在时间细胞周期上下文中，从而揭示了以前未知的组件和模块。我们发现大多数复合物都由定期和组成型表达的亚基组成，这表明以前的控制复合活动通过即时组装的机制。与此相一致，我们表明，通过CDC28P（CDK1）的靶向降解和磷酸化的额外调节特别影响了定期表达的蛋白质。
To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.