Distribution of pectin and arabinogalactan protein epitopes during organogenesis from androgenic callus of wheat
Distribution of pectin and arabinogalactan protein epitopes during organogenesis from androgenic callus of wheat
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DOI：
10.1007/s00299-006-0222-6
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发表时间：
2007-03-01
影响因子：
6.2
通讯作者：
Bohdanowicz, Jerzy
中科院分区：
文献类型：
--
作者：
Konieczny, Robert;Swierczynska, Joanna;Bohdanowicz, Jerzy
研究了几种阿拉伯半乳糖蛋白和果胶表位在小麦雄性愈伤组织器官发生过程中的分布。在成熟和退化薄壁细胞的细胞壁中，阿拉伯半乳糖表位JIM4、JIM14、JIM16或LM2的表达因细胞位置不同而不同。在再生芽和叶片的分生组织细胞中也观察到LM2。抗果胶JIM7标记分生组织细胞壁，但在细胞外基质表面网络(ECMSN)包被的愈伤组织和芽的表面细胞外壁荧光最强。在叶片生长过程中，ECMSN消失，JIM7荧光减弱。JIM5表位丰富存在于愈伤组织细胞间隙的细胞壁和再生芽、叶片的三细胞连接处。
The distribution of several arabinogalactan protein and pectic epitopes were studied during organogenesis in androgenic callus of wheat. In cell wall of mature and degenerating parenchyma cells, the arabinogalactan epitopes JIM4, JIM14, JIM16 or LM2 were expressed differently according to the cells location. LM2 was observed also in meristematic cells of regenerated shoot buds and leaves. Anti-pectin JIM7 labelled the wall of meristematic cells but fluorescence was strongest in outer walls of surface cells of callus and shoot buds coated by extracellular matrix surface network (ECMSN). During leaves growth the ECMSN disappeared, and JIM7 fluorescence decreased. JIM5 epitope was abundant in the cell walls lining the intercellular spaces of callus parenchyma and in tricellular junctions within regenerated buds and leaves.