CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN CDNA FOR THE ALPHA(1C)-ADRENERGIC RECEPTOR
CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN CDNA FOR THE ALPHA(1C)-ADRENERGIC RECEPTOR
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DOI：
10.1006/bbrc.1993.2130
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发表时间：
1993-09-15
影响因子：
3.1
通讯作者：
TSUJIMOTO, G
中科院分区：
文献类型：
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作者：
HIRASAWA, A;HORIE, K;TSUJIMOTO, G
我们从人前列腺cDNA文库中克隆了人α 1C肾上腺素能受体。该克隆（P2C4）的推导氨基酸序列编码一个466个氨基酸的蛋白质，与先前克隆的牛α 1C-肾上腺素能受体具有很强的序列同源性。COS-7细胞表达的P2C4与牛α 1C肾上腺素能受体的放射配基结合特性非常相似。用逆转录聚合酶链反应（RT-PCR）方法检测心、脑、肝和前列腺中α 1C-肾上腺素能受体的转录本，但在肾、肺、肾上腺、主动脉和垂体中未检测到α 1C-肾上腺素能受体转录本。结果表明，克隆P2C4编码人α 1C肾上腺素能受体cDNA，该受体亚型在几种人体组织中不广泛表达，但定位于几种人体组织中。
We have cloned human α1C-adrenergic receptor from human prostate cDNA library. The deduced amino acid sequence of the clone (P2C4) encodes a protein of 466 amino acids that showed strong sequence homology to the previously cloned bovine α1C-adrenergic receptor. The radioligand binding properties of P2C4 expressed in COS-7 cells were very similar to those of bovine α1C-adrenergic receptor. With reverse-transcription polymerase chain reaction assay, we observed α1C-adrenergic receptor transcripts in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. The data show that the clone P2C4 encodes a human α1C-adrenergic receptor cDNA, and the receptor subtype is expressed not widely but localized in several human tissues.