Two Fe-superoxide dismutase families respond differently to stress and senescence in legumes
Two Fe-superoxide dismutase families respond differently to stress and senescence in legumes
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DOI：
10.1016/j.jplph.2012.04.019
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发表时间：
2012-09-01
影响因子：
4.3
通讯作者：
Fernando Moran, Jose
中科院分区：
文献类型：
--
作者：
Asensio, Aaron C.;Gil-Monreal, Miriam;Fernando Moran, Jose
根据活性中心的金属，植物中的 SOD 可以分为三个主要家族：CuZnSOD、MnSOD 和 FeSOD。 CuZnSOD 有两个位于植物细胞胞浆或质体中的亚家族，MnSOD 家族基本上仅限于线粒体，而 FeSOD 酶家族通常位于质体中。在这里，我们根据系统发育树和实验数据描述了两个 FeSOD 亚家族的存在：植物中普遍存在的质体局部亚家族和在确定形成根瘤豆科植物中观察到的细胞质局部 FeSOD 亚家族。使用抗胞质 FeSOD (cyt_FeSOD) 抗体以及针对质体 FeSOD (p_FeSOD) 的新型抗体。胁迫条件，如硝酸盐过量或干旱，会显着增加大豆组织中的 cyt_FeSOD 含量。此外，大豆和豇豆植物中 cyt_FeSOD 的含量和活性随着年龄的增长而增加，而 cyt_CuZnSOD 同工酶在早期阶段占主导地位。叶片中的 p_FeSOD 随着大部分胁迫的施加而降低，但该同工酶随着根中脱落酸的存在而显着增加。在植物组织中观察到的 p_FeSOD 和 cyt_FeSOD 含量响应应激和衰老的巨大差异揭示了不同的功能，并证实了两个免疫分化的 FeSOD 亚家族的存在。凝胶内 FeSOD 活性模式显示与 cyt_FeSOD 含量有良好的相关性，但与 p_FeSOD 含量无关。这表明cyt_FeSOD是大豆和豇豆组织中主要的活性FeSOD。讨论了与 FeSOD 同工酶复杂性相关的功能多样性（取决于位置）。 (c) 2012 年爱思唯尔有限公司。版权所有。
Three main families of SODs in plants may be distinguished according to the metal in the active center: CuZnSODs, MnSOD, and FeSOD. CuZnSODs have two sub-families localized either in plant cell cytosol or in plastids, the MnSOD family is essentially restricted to mitochondria, and the FeSOD enzyme family has been typically localized into the plastid. Here, we describe, based on a phylogenetic tree and experimental data, the existence of two FeSOD sub-families: a plastidial localized sub-family that is universal to plants, and a cytosolic localized FeSOD sub-family observed in determinate-forming nodule legumes. Anti-cytosolic FeSOD (cyt_FeSOD) antibodies were employed, together with a novel antibody raised against plastidial FeSOD (p_FeSOD). Stress conditions, such as nitrate excess or drought, markedly increased cyt_FeSOD contents in soybean tissues. Also, cyt_FeSOD content and activity increased with age in both soybean and cowpea plants, while the cyt_CuZnSOD isozyme was predominant during early stages. p_FeSOD in leaves decreased with most of the stresses applied, but this isozyme markedly increased with abscisic acid in roots. The great differences observed for p_FeSOD and cyt_FeSOD contents in response to stress and aging in plant tissues reveal distinct functionality and confirm the existence of two immunologically differentiated FeSOD sub-families. The in-gel FeSOD activity patterns showed a good correlation to cyt_FeSOD contents but not to those of p_FeSOD. This indicates that cyt_FeSOD is the main active FeSOD in soybean and cowpea tissues. The diversity of functions associated with the complexity of FeSOD isoenzymes depending of the location is discussed. (c) 2012 Elsevier GmbH. All rights reserved.