Ablation of synovial pannus using microbubble-mediated ultrasonic cavitation in antigen-induced arthritis in rabbits
Ablation of synovial pannus using microbubble-mediated ultrasonic cavitation in antigen-induced arthritis in rabbits
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DOI：
10.1007/s00296-011-2285-0
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发表时间：
2012-12-01
影响因子：
4
通讯作者：
Luo, Yan
中科院分区：
文献类型：
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作者：
Qiu, Li;Jiang, Yong;Luo, Yan
研究微泡介导的超声空化治疗滑膜血管翳的消融有效性，并使用抗原诱导的关节炎模型（AIA）确定潜在的机制。采用最佳超声消融参数对AIA兔膝关节进行超声消融。将抗原诱导性关节炎家兔随机分为4组：（1）超声（US）+微泡组;（2）仅超声组;（3）仅微泡组;（4）对照组。分别于首次消融后1 h和14 d行超声造影（CEUS）监测和病理学滑膜炎评分评价疗效。还测量了滑膜坏死和微血管变化。消融治疗后，超声造影测量滑膜厚度、时间-强度曲线参数（峰值强度和曲线下面积），超声+微泡组病理滑膜炎评分明显低于其余各组。周围正常组织未见损伤。超声消融的机制与微血栓形成和微血管破裂导致滑膜坏死有关。结果表明，微泡介导的超声空化应适用于作为一种非侵入性的策略，在最佳条件下治疗关节炎滑膜血管翳。
To investigate the ablative effectiveness of microbubble-mediated ultrasonic cavitation for treating synovial pannus and to determine a potential mechanism using the antigen-induced arthritis model (AIA). Ultrasonic ablation was performed on the knee joints of AIA rabbits using optimal ultrasonic ablative parameters. Rabbits with antigen-induced arthritis were randomly assigned to 4 groups: (1) the ultrasound (US) + microbubble group; (2) the US only group; (3) the microbubble only group, and (4) the control group. At 1 h and 14 days after the first ablation, contrast-enhanced ultrasonography (CEUS) monitoring and pathology synovitis score were used to evaluate the therapeutic effects. Synovial necrosis and microvascular changes were also measured. After the ablation treatment, the thickness of synovium and parameters of time intensity curve including derived peak intensity and area under curve were measured using CEUS, and the pathology synovitis score in the ultrasound + microbubble group was significantly lower than that found in the remaining groups. No damage was observed in the surrounding normal tissues. The mechanism underlying the ultrasonic ablation was related to microthrombosis and microvascular rupture that resulted in synovial necrosis. The results suggest that microbubble-mediated ultrasonic cavitation should be applied as a non-invasive strategy for the treatment of synovial pannus in arthritis under optimal conditions.