Effects of Chronic Whey Protein Supplementation on Atherosclerosis in ApoE-/- Mice.
Effects of Chronic Whey Protein Supplementation on Atherosclerosis in ApoE-/- Mice.
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DOI：
10.3177/jnsv.64.143
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发表时间：
2018
影响因子：
1.6
通讯作者：
Zheng Zhang;Ru Zhang;Zhi-Zhen Qin;Jia-Ping Chen;Jia-Ying Xu;L. Qin
中科院分区：
文献类型：
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作者：
Zheng Zhang;Ru Zhang;Zhi-Zhen Qin;Jia-Ping Chen;Jia-Ying Xu;L. Qin
乳清蛋白与代谢综合征的改善有关。本研究旨在评价乳清蛋白对ApoE-/-小鼠动脉粥样硬化的影响。雄性ApoE-/-小鼠被喂食高脂肪/胆固醇饮食（HFCD），或HFCD中添加10%或20%乳清蛋白，持续18周。实验结束时测定血清脂质和炎症因子。采用HE染色和油红O染色检测肝脏。主动脉表面及冰冻切片观察主动脉病变情况。Western blotting分析肝、主动脉中胆固醇代谢的相对蛋白表达。三组患者的体重和食物摄入量均无显著差异。肝脏检查显示，乳清蛋白补充组的脂滴和胆固醇含量降低。添加10%和20%乳清的HFCD组，主动脉表面病变分别减少21.51%和31.78%。在冷冻切片分析中也观察到病变减少。乳清蛋白显著提高血清高密度脂蛋白胆固醇水平，分别提高46.43%和67.86%。20%乳清蛋白显著降低血清IL-6（促炎细胞因子）70.99%，显著升高血清IL-10（抗炎细胞因子）83.35%。乳清蛋白能显著降低肝脏脂肪生成酶（ACC和FAS）及肝脏和主动脉NF-κB的表达。乳清蛋白显著增加肝脏和主动脉中两种主要胆固醇转运蛋白（ABCA1和ABCG1）的蛋白表达。因此，长期补充乳清蛋白可以通过调节循环脂质和炎症细胞因子以及增加ABCA1和ABCG1的表达来改善ApoE缺失小鼠hfcd诱导的动脉粥样硬化。
Whey protein is associated with improvement of metabolic syndrome. This study aimed to evaluate effects of whey protein on atherosclerosis in ApoE-/- mice. Male ApoE-/- mice were fed with a high-fat/cholesterol diet (HFCD), or HFCD supplemented with 10% or 20% whey protein for 18 wk. At the end of experiment, serum lipid profiles and inflammatory cytokines were assayed. Livers were examined using HE staining and Oil Red O staining. Aortas were used for en face and cryosection analyses to observe aortic lesions. Western blotting analysis was used to assess relative protein expression of cholesterol metabolism in the liver and aorta. No significant differences were observed in body weight or food intake among the three groups. Liver examination demonstrated decreased lipid droplets and cholesterol content in the whey-protein-supplemented groups. En face lesion of the aorta revealed a 21.51% and 31.78% lesion reduction in the HFCD supplemented with 10% and 20% whey groups, respectively. Decreased lesion was also observed in cryosection analysis. Whey protein significantly increased the serum high-density lipoprotein cholesterol level by 46.43% and 67.86%. The 20% whey protein significantly decreased serum IL-6 (a proinflammatory cytokine) by 70.99% and increased serum IL-10 (an anti-inflammatory cytokine) by 83.35%. Whey protein potently decreased lipogenic enzymes (ACC and FAS) in the liver and NF-κB expression in the liver and aorta. Whey protein significantly increased protein expression of two major cholesterol transporters (ABCA1 and ABCG1) in the liver and aorta. Thus, chronic whey protein supplementation can improve HFCD-induced atherosclerosis in ApoE null mice by regulating circulating lipid and inflammatory cytokines and increasing expressions of ABCA1 and ABCG1.