Survivability of Immunoassay Reagents Exposed to the Space Radiation Environment on board the ESA BIOPAN-6 Platform as a Prelude to Performing Immunoassays on Mars
Survivability of Immunoassay Reagents Exposed to the Space Radiation Environment on board the ESA BIOPAN-6 Platform as a Prelude to Performing Immunoassays on Mars
复制标题
作为在火星上进行免疫测定的前奏，ESA BIOPAN-6 平台上暴露于空间辐射环境的免疫测定试剂的生存能力
DOI：
10.1089/ast.2012.0871
复制
发表时间：
2013
期刊：
影响因子：
4.2
通讯作者：
Derveni M
中科院分区：
文献类型：
--
作者：
Derveni M
生命标记芯片（LMC）仪器是一种基于免疫测定的传感器，它将试图探测火星地表下的生命特征。LMC核心的分子试剂没有行星际使命使用的传统;因此，这种仪器的设计必须考虑到一些风险因素，包括在火星使命期间将遇到的辐射环境。为了研究空间辐射对免疫测定试剂（主要是抗体）的影响，在欧洲空间局2007年的BIOPAN-6低地球轨道空间照射平台上进行了一项空间研究，以补充一系列地面辐射研究。研究中使用了两种抗体，将其冻干并以预期的LMC形式包装，并加载到安装在BIOPAN-6平台上的定制样品保持器单元中。BIOPAN使命进入低地球轨道12天，之后回收了所有样品，并通过酶联免疫吸附测定法测量了抗体结合性能。预计影响抗体性能的因素是空间使命的物理条件和暴露于空间条件，主要是低地球轨道的辐射环境。这两种抗体在这些因素的灭活下存活下来，这是从飞行样品与许多运输和储存对照之间的比较中得出的结论。这项工作与对代表性LMC抗体进行的地面辐射测试相结合，有助于降低在行星探索使命中使用抗体的风险。关键词：近地轨道-辐射-生命探测仪器-航天-火星。Astrobiology 13，92-102.
The Life Marker Chip (LMC) instrument is an immunoassay-based sensor that will attempt to detect signatures of life in the subsurface of Mars. The molecular reagents at the core of the LMC have no heritage of interplanetary mission use; therefore, the design of such an instrument must take into account a number of risk factors, including the radiation environment that will be encountered during a mission to Mars. To study the effects of space radiation on immunoassay reagents, primarily antibodies, a space study was performed on the European Space Agency's 2007 BIOPAN-6 low-Earth orbit (LEO) space exposure platform to complement a set of ground-based radiation studies. Two antibodies were used in the study, which were lyophilized and packaged in the intended LMC format and loaded into a custom-made sample holder unit that was mounted on the BIOPAN-6 platform. The BIOPAN mission went into LEO for 12 days, after which all samples were recovered and the antibody binding performance was measured via enzyme-linked immunosorbent assays (ELISA). The factors expected to affect antibody performance were the physical conditions of a space mission and the exposure to space conditions, primarily the radiation environment in LEO. Both antibodies survived inactivation by these factors, as concluded from the comparison between the flight samples and a number of shipping and storage controls. This work, in combination with the ground-based radiation tests on representative LMC antibodies, has helped to reduce the risk of using antibodies in a planetary exploration mission context. Key Words: Low-Earth orbit—Radiation—Life-detection instruments—Spaceflight—Mars. Astrobiology 13, 92–102.