A glycine-dependent riboswitch that uses cooperative binding to control gene expression
A glycine-dependent riboswitch that uses cooperative binding to control gene expression
复制标题
DOI：
10.1126/science.1100829
复制
发表时间：
2004-10-08
期刊：
影响因子：
56.9
通讯作者：
Breaker, RR
中科院分区：
文献类型：
--
作者：
Mandal, M;Lee, M;Breaker, RR
我们在细菌中发现了一种以前未知的核糖开关类别，它是由甘氨酸选择性触发的。这些来自枯草芽孢杆菌的甘氨酸感应 RNA 的代表作为 gcvT 操纵子的罕见遗传开关发挥作用，该操纵子编码形成甘氨酸裂解系统的蛋白质。大多数甘氨酸核糖开关整合了两个配体结合域，它们协同作用以更接近于两种状态的遗传开关。这种先进形式的核糖开关可能已经进化，以确保过量的甘氨酸有效地用于通过柠檬酸循环提供碳通量，并维持足够量的氨基酸用于蛋白质合成。因此，核糖开关发挥着关键的调节作用，并表现出以前仅在蛋白质因子中观察到的复杂性能特征。
We identified a previously unknown riboswitch class in bacteria that is selectively triggered by glycine. A representative of these glycine-sensing RNAs from Bacillus subtilis operates as a rare genetic on switch for the gcvT operon, which codes for proteins that form the glycine cleavage system. Most glycine riboswitches integrate two ligand-binding domains that function cooperatively to more closely approximate a two-state genetic switch. This advanced form of riboswitch may have evolved to ensure that excess glycine is efficiently used to provide carbon flux through the citric acid cycle and maintain adequate amounts of the amino acid for protein synthesis. Thus, riboswitches perform key regulatory roles and exhibit complex performance characteristics that previously had been observed only with protein factors.