Coat color-tagged green mouse with EGFP expressed from the RNA polymerase II promoter
Coat color-tagged green mouse with EGFP expressed from the RNA polymerase II promoter
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DOI：
10.1002/gene.20038
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发表时间：
2004-06-01
期刊：
影响因子：
1.5
通讯作者：
Tsai, TF
中科院分区：
文献类型：
--
作者：
Hsiao, YC;Chang, HH;Tsai, TF
费力的分子基因分型和多样化的基因表达是转基因小鼠研究中广泛遇到的两个问题。为了促进FVB/N白化背景中的基因分型并减少杂色表达，我们成功地产生了用于直接视觉基因分型的双标记转基因小鼠，其毛色表型源自酪氨酸酶启动子驱动的酪氨酸酶cDNA，并且同时具有由RNA聚合酶II大亚基基因的启动子驱动的高增强型绿色荧光蛋白（EGFP）表达。将绝缘体掺入转基因构建体中，超过 90% 的创始人实现了转基因高效率表达。 EGFP 早在单细胞受精卵时就被检测到，并持续到整个胚胎发育以及所有检查的成体组织中。毛色标记的绿色小鼠为组织移植、谱系追踪、嵌合体生物学、RNA干扰和其他转基因研究等应用提供了机会。 (C) 2004 Wiley-Liss, Inc.
Laborious molecular genotyping and variegated gene expression are two widely encountered issues for transgenic mouse studies. To facilitate genotyping in the FVB/N albino background and to reduce variegated expression, we successfully generated double-tagged transgenic mice for direct visual genotyping with the coat color phenotype derived from tyrosinase cDNA driven by the tyrosinase promoter and with simultaneous high enhanced green fluorescent protein (EGFP) expression driven by the promoter of RNA polymerase II large subunit gene. Incorporation of insulator into a transgene construct achieved high efficiency of transgene expression in more than 90% of the founders. EGFP was detected as early as the one-cell fertilized egg and lasted for the whole embryo development, as well as in all of the adult tissues examined. The coat color-tagged green mice offer opportunities in applications such as tissue transplantation, lineage tracing, chimera biology, RNA interference, and other transgenic studies. (C) 2004 Wiley-Liss, Inc.