Retention of proteins and metalloproteins in open tubular capillary electrochromatography with etched chemically modified columns.
Retention of proteins and metalloproteins in open tubular capillary electrochromatography with etched chemically modified columns.
复制标题
使用蚀刻化学改性柱在开管毛细管电色谱中保留蛋白质和金属蛋白质。
DOI：
10.1002/elps.200800142
复制
发表时间：
2008
期刊：
影响因子：
2.9
通讯作者：
Salgotra,Vasudha
中科院分区：
文献类型：
--
作者：
Pesek,JosephJ;Matyska,MariaT;Salgotra,Vasudha
比较了具有两种不同键合基团（戊基和十八烷基）的化学修饰毛细管对几种常见蛋白质和金属蛋白以及金属蛋白酶的迁移行为。在2.1-8.1的pH范围内评价迁移时间、效率和峰形，以确定键合基团对这些化合物的电色谱行为的任何影响。一个目标是确定固定相的相对疏水性是否像在HPLC中那样对毛细管电色谱的开放管状形式中的蛋白质具有显著影响。迁移时间的再现性也进行了研究。
Etched chemically modified capillaries with two different bonded groups (pentyl and octadecyl) are compared for their migration behavior of several common proteins and metalloproteins as well as metalloproteinases. Migration times, efficiency and peak shape are evaluated over the pH range of 2.1–8.1 to determine any effects of the bonded group on the electrochromatographic behavior of these compounds. One goal was to determine if the relative hydrophobicity of the stationary phase has a significant effect on proteins in the open tubular format of capillary electrochromatography as it does in HPLC. Reproducibility of the migration times is also investigated.