Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube
Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube
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DOI：
10.1101/gad.559910
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发表时间：
2010-06-01
影响因子：
10.5
通讯作者：
Briscoe, James
中科院分区：
文献类型：
--
作者：
Ribes, Vanessa;Balaskas, Nikolaos;Briscoe, James
分泌的配体Sonic Hedgehog（Shh）组织腹侧神经管中的细胞分化模式。对于五种神经元亚型，增加Shh信号传导的水平和持续时间将祖细胞引导到逐渐更腹侧的身份。在这里，我们证明，这种模式的行动是不适用于最腹侧的细胞类型，非神经元底板（FP）的代。在鸡和小鼠胚胎中，FP特化涉及对Shh信号的双相反应，其控制关键转录因子的动态表达。在原肠胚形成和早期体节发生期间，FP诱导依赖于高水平的Shh信号传导。然而，随后，预期的FP细胞变得对Shh信号传导不敏感，这是阐述其身份的先决条件。这提示了对神经管中分级Shh信号传导模型的修订，并提供了对形态发生素信号传导的动态如何部署以扩展单个配体的图案化能力的见解。此外，我们提供的证据支持一个共同的计划FP规格的Shh信号，调和机制FP发展硬骨鱼和鱼。
The secreted ligand Sonic Hedgehog (Shh) organizes the pattern of cellular differentiation in the ventral neural tube. For the five neuronal subtypes, increasing levels and durations of Shh signaling direct progenitors to progressively more ventral identities. Here we demonstrate that this mode of action is not applicable to the generation of the most ventral cell type, the nonneuronal floor plate (FP). In chick and mouse embryos, FP specification involves a biphasic response to Shh signaling that controls the dynamic expression of key transcription factors. During gastrulation and early somitogenesis, FP induction depends on high levels of Shh signaling. Subsequently, however, prospective FP cells become refractory to Shh signaling, and this is a prerequisite for the elaboration of their identity. This prompts a revision to the model of graded Shh signaling in the neural tube, and provides insight into how the dynamics of morphogen signaling are deployed to extend the patterning capacity of a single ligand. In addition, we provide evidence supporting a common scheme for FP specification by Shh signaling that reconciles mechanisms of FP development in teleosts and amniotes.