Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid
Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid
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DOI：
10.1007/s001220051549
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发表时间：
2000-10-01
影响因子：
5.4
通讯作者：
Zhang, QF
中科院分区：
文献类型：
--
作者：
Tan, YF;Xing, YZ;Zhang, QF
稻米外观品质是世界上许多水稻产区，特别是我国杂交水稻生产中的一个主要问题。在这项研究中，我们进行了基于分子标记的遗传分析的性状是决定外观品质的水稻籽粒，包括性状指定的粒形和胚乳不透明度。分析所用材料包括来自中国种植最广泛的杂交水稻汕优63亲本间杂交的F-2：3群体和F-10重组自交系群体。基于分子标记的QTL（quantitative trait locus，数量性状位点）分析表明，粒长和粒宽分别受一个主效QTL和一个或两个次效QTL控制，主效QTL占遗传变异的很大比例，次效QTL占遗传变异的很小比例。在水稻和糙米的F-2：3和重组自交系群体中均检测到主效QTL，而在重组自交系群体中仅偶尔检测到次效QTL。位于第3染色体RG 393-C1087区间的QTL是决定粒长的主效位点，位于第5染色体RG 360-C734 a区间的QTL是决定粒宽的主效位点。同样，白色腹，这在很大程度上决定了胚乳的不透明度，几乎完全由第5染色体上的一个主基因座控制，该主基因座位于与粒宽主QTL相同的基因组区域。最后讨论了这一结果对杂交水稻改良的意义。
Appearance quality of the rice grain represents a major problem of rice production in many rice-producing areas of the world, especially in hybrid rice production in China. In this study, we conducted a molecular marker-based genetic analysis of the traits that are determinants of the appearance quality of rice grains, including traits specifying grain shape and endosperm opacity. The materials used in the analysis included an F-2:3 population and an F-10 recombinant inbred line population from a cross between the parents of Shanyou 63, the most widely grown rice hybrid in China. Molecular marker-based QTL (quantitative trait locus) analyses revealed that grain length and grain width were each controlled by a major QTL accounting for a very large proportion of the genetic variation, plus one or two minor QTLs each explaining a small proportion of the genetic variation. The major QTLs can be detected in both the F-2:3 and recombinant inbred line population using both paddy rice and brown rice, whereas the minor QTLs were detected only occasionally. The QTL located in the interval of RG393-C1087 on chromosome 3 is the major locus for grain length, and the one in the interval RG360-C734a on chromosome 5 plays a major role in determining grain width. Similarly, white belly, which largely determines the opacity of the endosperm, is almost entirely controlled by a major locus on chromosome 5, located in the same genomic region as the major QTL for grain width. The implications of the results with respect to hybrid rice improvement were discussed.