The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro
The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro
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DOI：
10.1111/rda.12067
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发表时间：
2012-12-01
影响因子：
1.7
通讯作者：
Wildt, D. E.
中科院分区：
文献类型：
--
作者：
Songsasen, N.;Comizzoli, P.;Wildt, D. E.
内容卵泡培养是了解卵泡发育和卵母细胞分化机制的重要工具。从早期卵泡（原始卵泡、初级卵泡、前窦卵泡和早期窦卵泡）中恢复减数分裂和发育能力的卵母细胞的益处也将是显著的，从拯救濒危物种的基因组到保护面临癌症治疗的妇女的生育能力。这个研究领域处于科学发现的早期阶段。迄今为止，从培养的原始卵泡产生可受精卵母细胞的活后代只发生在小鼠中。在其他更复杂的物种中的进展受到限制，因为较大的动物具有更长的自然卵泡形成持续时间，从而需要更多的培养时间来产生完全生长的卵泡和卵母细胞。我们相信狗和猫是了解体外卵泡发生的极好模型。这篇综述强调了这两个物种对这一主题的了解以及未来的优先事项。我们已经发现，这是更具有挑战性的保持原始卵泡的活力在卵巢组织中的狗比猫在体外。尽管如此，它是可能的，以培养分离的猫和狗腔前卵泡。虽然这两个物种的卵泡都有能力增加体积并产生类固醇，但只有猫的卵母细胞形态正常。这两个物种之间的这种显着差异的原因是一个高度优先研究的领域。虽然需要更多的基础数据，但我们设想了先进的技术，可以从肉食动物卵巢中大量未使用的卵泡储存中收获卵母细胞。这些配子可用于繁殖具有遗传价值的狗和猫，这些狗和猫是研究人类疾病的伴侣或生物医学模型，以及用于挽救在为遗传管理计划做出贡献之前死亡的稀有犬科动物和猫科动物物种的基因组。
Contents The culture of ovarian follicles is an important tool for understanding the mechanisms controlling follicle development and differentiation of the oocyte. The benefit of recovering meiotically and developmentally competent oocytes from early stage follicles (primordial, primary, pre-antral and early antral) also would be significant, ranging from rescue of genomes from endangered species to preserving fertility in women facing cancer treatments. This research field is at an early stage of scientific discovery. To-date, live offspring from cultured primordial follicles that produced fertilizable oocytes has occurred only in the mouse. Progress in other more complex species has been limited because larger animals have longer durations of natural folliculogenesis, thereby requiring more culture time to generate fully grown follicles and oocytes. We believe the dog and cat are excellent models for understanding more about folliculogenesis in vitro. This review highlights what is known about this topic for these two species as well as future priorities. We have discovered that it is more challenging to maintain viability of primordial follicles within ovarian tissues in vitro in the dog than the cat. Nonetheless, it is possible to grow both isolated cat and dog pre-antral follicles in culture. Although the follicles of both species have the capacity to increase in size and produce steroids, only cat oocytes appear morphologically normal. The reason for this striking difference between these two species is an area of high research priority. While much more fundamental data are required, we envision advanced technology that will allow harvesting oocytes from the vast, unused follicle stores sequestered within carnivore ovaries. These gametes have utility for reproducing genetically valuable dogs and cats that are companions or biomedical models for investigating human disorders as well as for salvaging the genomes of rare canid and felid species that die before contributing to genetic management programs.