COLOCALIZATION OF THROMBOSPONDIN AND SYNDECAN DURING MURINE DEVELOPMENT
COLOCALIZATION OF THROMBOSPONDIN AND SYNDECAN DURING MURINE DEVELOPMENT
复制标题
DOI：
10.1002/aja.1001930408
复制
发表时间：
1992-04-01
影响因子：
2.5
通讯作者：
LAWLER, J
中科院分区：
文献类型：
--
作者：
CORLESS, CL;MENDOZA, A;LAWLER, J
血小板反应蛋白是一种粘附糖蛋白，被认为在组织发生和修复中发挥作用。 我们使用了单克隆抗血小板反应蛋白抗体（命名为 5G11），将血小板反应蛋白定位在发育中小鼠胚胎的多聚甲醛固定、石蜡包埋的切片中。 在胚胎植入后发育的初始阶段，在子宫平滑肌、子宫内膜腺、蜕膜和滋养层巨细胞中观察到血小板反应蛋白表达。 妊娠第 8.5 天，心肌细胞和神经上皮细胞显示血小板反应蛋白阳性染色，并且这种表达在心肌和中枢神经系统的整个发育过程中持续存在。 在发育中的骨骼和肝脏中可以看到血小板反应蛋白的强烈染色。 还在发育中的平滑肌和骨骼肌以及多种上皮中观察到血小板反应蛋白，包括表皮、小肠上皮、晶状体上皮、肾小管上皮和发育中牙齿的上皮。 将血小板反应蛋白染色与两种已知的血小板反应蛋白细胞表面受体（多配体聚糖和玻连蛋白受体）的染色进行比较，揭示了血小板反应蛋白和多配体聚糖在所有组织中的显着共定位，但几乎不与玻连蛋白受体共表达。 血小板反应蛋白和多聚糖的共表达可能在发育过程中的细胞-细胞或细胞-基质相互作用中发挥重要作用。
Thrombospondin is an adhesive glycoprotein that is thought to play a role in tissue genesis and repair. We have used a monoclonal anti-thrombospondin antibody, designated 5G11, to localize thrombospondin in paraformaldehyde fixed, paraffin-embedded sections of developing mouse embryos. Thrombospondin expression is observed in uterine smooth muscle, endometrial glands, the decidua, and trophoblastic giant cells during the initial phase of post-implantation development in the embryo. Cardiac myocytes and neuroepithelial cells show positive staining for thrombospondin at day 8.5 of gestation, and this expression continues throughout the development of the myocardium and central nervous system. Strong staining for thrombospondin is seen in developing bone and in the liver. Thrombospondin is also observed in developing smooth muscle and skeletal muscle, as well as in a variety of epithelia, including the epidermis, small intestinal epithelium, lens epithelium, renal tubular epithelium, and the epithelium of the developing tooth. Comparison of thrombospondin staining with that of two known cell surface receptors for thrombospondin, syndecan and the vitronectin receptor, reveals remarkable colocalization of thrombospondin and syndecan in all tissues, but almost no coexpression with the vitronectin receptor. Coexpression of thrombospondin and syndecan may play an important role in cell-cell or cell-matrix interactions during development.