Simultaneous determination of pilocarpine and isopilocarpine in pharmaceutical preparations by liquid chromatography.
Simultaneous determination of pilocarpine and isopilocarpine in pharmaceutical preparations by liquid chromatography.
复制标题
液相色谱法同时测定药物制剂中毛果芸香碱和异毛果芸香碱的含量。
DOI：
10.1002/jps.2600650220
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发表时间：
1976
影响因子：
3.8
通讯作者：
G. Manning
中科院分区：
文献类型：
--
作者：
T. Urbanyi;A. Piedmont;E. Willis;G. Manning
建立了同时测定药物制剂中毛果芸香碱和异毛果芸香碱含量的液相色谱法。该方法包括分离的异构体上的高分辨率离子交换柱，然后通过检测毛果芸香碱和异洛果芸香碱的紫外吸收在217 nm区域。该方法的专属性使得毛果芸香碱和异毛果芸香碱可以在彼此存在下以及在市售毛果芸香碱溶液中常见的辅料存在下分别测定。它对异毛果芸香碱的灵敏度至少为25 ppm（与毛果芸香碱的浓度无关）。相比之下，USP方法缺乏特异性和灵敏度。结论：本法适用于毛果芸香碱市售制剂的常规分析，是对官方方法的改进。
A liquid chromatographic assay method is described for the simultaneous determination of pilocarpine and isopilocarpine in pharmaceutical preparations. The method involves separation of the isomers on a high-resolution ion-exchange column, followed by detection of pilocarpine and isopilocarpine by UV absorption in the 217-nm region. The specificity of the method is such that pilocarpine and isopilocarpine can be assayed separately in the presence of one another and in the presence of the excipients commonly found in commercial pilocarpine solutions. Its sensitivity for isopilocarpine is at least 25 ppm (independent of the concentration of pilocarpine). In contrast, USP methods lack both specificity and sensitivity. It is concluded that this method is applicable to the routine analysis of commercial pilocarpine preparations and is an improvement over the official methods.