Classic and 11-oxygenated androgens in serum and saliva across adulthood: a cross-sectional study analyzing the impact of age, body mass index, and diurnal and menstrual cycle variation.
Classic and 11-oxygenated androgens in serum and saliva across adulthood: a cross-sectional study analyzing the impact of age, body mass index, and diurnal and menstrual cycle variation.
复制标题
成年期血清和唾液中的经典雄激素和 11 氧化雄激素：一项横断面研究，分析年龄、体重指数以及昼夜和月经周期变化的影响。
DOI：
10.1093/ejendo/lvac017
复制
发表时间：
2023
影响因子：
5.8
通讯作者：
Schiffer L
中科院分区：
文献类型：
--
作者：
Schiffer L
β 11-氧合雄激素对循环雄激素库有显著贡献。了解11-氧合雄激素及其决定因素的生理变化是至关重要的临床解释，例如，在雄激素过剩的条件。我们在成人年龄和体重指数（BMI）范围内定量了血清和唾液中的经典和11-氧合雄激素，还分析了昼夜和月经周期依赖性变化。从290名健康志愿者（125名男性，22-95岁; 165名女性，21-91岁）中收集早晨血清样品。早晨唾液样本由一个亚组（51名女性和32名男性）收集。收集了13名男性的昼夜唾液曲线。十二名妇女收集昼夜唾液配置文件和早晨唾液样本连续7天在卵泡和黄体月经周期phase.MethodsSerum和唾液类固醇进行了定量液相色谱串联质谱profiling assessment.ResultsSerum经典雄激素降低与年龄调整后的BMI，例如，%变化kg/m2为5α-双氢睾酮：男性−5.54%（95%置信区间（CI）−8.10至−2.98）和女性−1.62%（95%CI −3.16至−0.08）。 相比之下，11-氧合雄激素随着BMI增加而增加，例如，11-酮睾酮的变化% kg/m2：男性3.05%（95%CI 0.08-6.03）和女性1.68%（95%CI −0.44至3.79）。 相反，男性和女性的经典雄激素随着年龄的增长而减少，而11-氧合雄激素则没有。唾液中的雄激素表现出昼夜模式的男性和在卵泡期的妇女，在黄体期，只有11-氧合雄激素表现出昼夜variation.ConclusionsClassic雄激素减少，而积极的11-氧合雄激素增加BMI的增加，指向脂肪组织质量的重要性，为11-氧合雄激素的激活。经典的但不是11-氧合的雄激素随着年龄的增长而下降。
Objective11-oxygenated androgens significantly contribute to the circulating androgen pool. Understanding the physiological variation of 11-oxygenated androgens and their determinants is essential for clinical interpretation, for example, in androgen excess conditions. We quantified classic and 11-oxygenated androgens in serum and saliva across the adult age and body mass index (BMI) range, also analyzing diurnal and menstrual cycle-dependent variation.DesignCross-sectional. Morning serum samples were collected from 290 healthy volunteers (125 men, 22-95 years; 165 women, 21-91 years). Morning saliva samples were collected by a sub-group (51 women and 32 men). Diurnal saliva profiles were collected by 13 men. Twelve women collected diurnal saliva profiles and morning saliva samples on 7 consecutive days during both follicular and luteal menstrual cycle phases.MethodsSerum and salivary steroids were quantified by liquid chromatography–tandem mass spectrometry profiling assays.ResultsSerum classic androgens decreased with age-adjusted BMI, for example, %change kg/m2for 5α-dihydrotestosterone: men −5.54% (95% confidence interval (CI) −8.10 to −2.98) and women −1.62% (95%CI −3.16 to −0.08). By contrast, 11-oxygenated androgens increased with BMI, for example, %change kg/m2for 11-ketotestosterone: men 3.05% (95%CI 0.08-6.03) and women 1.68% (95%CI −0.44 to 3.79). Conversely, classic androgens decreased with age in both men and women, while 11-oxygenated androgens did not. Salivary androgens showed a diurnal pattern in men and in the follicular phase in women; in the luteal phase, only 11-oxygenated androgens showed diurnal variation.ConclusionsClassic androgens decrease while active 11-oxygenated androgens increase with increasing BMI, pointing toward the importance of adipose tissue mass for the activation of 11-oxygenated androgens. Classic but not 11-oxygenated androgens decline with age.