Different modes of stimuli delivery elicit changes in glutamate driven, experience-dependent interneuron response in C. elegans
Different modes of stimuli delivery elicit changes in glutamate driven, experience-dependent interneuron response in C. elegans
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DOI：
10.1016/j.neures.2022.10.004
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发表时间：
2022-12-30
影响因子：
2.9
通讯作者：
Kunitomo, Hirofumi
中科院分区：
文献类型：
--
作者：
Mabardi, Llian;Sato, Hirofumi;Kunitomo, Hirofumi
与记忆相关的神经元反应通常由再现先前经验的感觉刺激引起。尽管这种感觉输入处理的重要性，其潜在的机制仍然知之甚少。秀丽隐杆线虫在食物存在下对盐浓度的趋化作用。两栖类感觉神经元ASE-左和ASE-右以相反的方式对环境盐浓度的增加和减少作出反应。AIA、AIB和AIY中间神经元位于ASE对的突触后，并被认为参与处理从ASE传输的盐信息。然而，它仍然是难以捉摸的，这些中间神经元的反应是如何调节刺激模式。在这里，我们表明，AIY中间神经元显示经验依赖性的反应逐渐盐浓度的变化，但不是突然逐步浓度的变化。具有AIY完整（但AIA和AIB消融）的动物在低盐培养后对低盐浓度的化学趋化与野生型动物相似。ASE神经元通过盐能信号传递有关环境的盐信息，指导中间神经元AIY的活动，促进向有利条件运动。
Memory-related neuronal responses are often elicited by sensory stimuli that recapitulate previous experience. Despite the importance of this sensory input processing, its underlying mechanisms remain poorly understood. Caenorhabditis elegans chemotax towards salt concentrations experienced in the presence of food. The amphid sensory neurons ASE-left and ASE-right respond to increases and decreases of ambient salt concentration in opposite manners. AIA, AIB and AIY interneurons are post-synaptic to the ASE pair and are thought to be involved in the processing of salt information transmitted from ASE. However, it remains elusive how the responses of these interneurons are regulated by stimulus patterns. Here we show that AIY interneurons display an experience-dependent response to gradual salt concentration changes but not to abrupt stepwise concentration changes. Animals with AIY intact (but AIA and AIB ablated) chemotax towards low salt concentrations similarly to wild-type animals after cultivation with low salt. ASE neurons transmit salt information about the environment through glutamatergic signaling, directing the activity of the interneurons AIY that promote movement towards favorable conditions.