ANTI-TUMOR EFFECTS OF EPHEDRINE AND ANISODAMINE ON SKBR3 HUMAN BREAST CANCER CELL LINE
ANTI-TUMOR EFFECTS OF EPHEDRINE AND ANISODAMINE ON SKBR3 HUMAN BREAST CANCER CELL LINE
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DOI：
10.21010/ajtcam.v13i1.4
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Dan Chen;Feng Ma;Xiao-hui Liu;Rui Cao;Xiong-Zhi Wu
中科院分区：
文献类型：
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作者：
Dan Chen;Feng Ma;Xiao-hui Liu;Rui Cao;Xiong-Zhi Wu
背景：探讨麻黄碱和山莨菪碱对人乳腺癌增殖的影响。材料和方法：分别用麻黄碱和/或山莨菪碱处理SKBR3细胞。台盼蓝排斥法测定细胞数量。流式细胞术检测细胞周期分布及凋亡情况。酶联免疫吸附法测定cAMP和细胞周期蛋白D1的浓度。Western blot法测定PKA。结果：麻黄碱和山莨菪碱抑制细胞增殖，阻滞SKBR3细胞于G0/G1期。麻黄碱和山莨菪碱增加了SKBR3细胞中CD1的水平。此外，在麻黄碱和山莨菪碱处理的SKBR3细胞中，细胞内cAMP浓度发生了显著变化。麻黄碱和山莨菪碱处理48小时后，PKA底物的磷酸化未被激活。结论：麻黄碱和山莨菪碱通过显著改变细胞内cAMP浓度抑制SKBR3细胞的增殖。
Background: To investigate the effects of ephedrine and anisodamine on the proliferation of human breast cancer. Materials and Methods: SKBR3 cell was treated with or without ephedrine and / or anisodamine, respectively. The trypan blue exclusion assay was used to determine cell numbers. Flow cytometry was used to assess cell cycle distribution and apoptosis. The concentration of cAMP and cyclin D1 was analyzed by enzyme-linked immunosorbent assay. Western blot was used to measure PKA. Results: Ephedrine and anisodamine inhibited cell proliferation and arrested SKBR3 cells at G0/G1 phases. Ephedrine and anisodamine increased the level of CD1 in SKBR3 cells. Furthermore, significant change in intracellular cAMP concentration was found in SKBR3 cells treated with ephedrine and anisodamine. The phosphorylation of PKA substrate was not activated after 48 hours of treatment with ephedrine and anisodamine. Conclusion: Ephedrine and anisodamine inhibit the proliferation of SKBR3 cells via a significantly change of intracellular cAMP concentration.