Arbuscular mycorrhizal fungi differ in affecting the flowering of a host plant under two soil phosphorus conditions
Arbuscular mycorrhizal fungi differ in affecting the flowering of a host plant under two soil phosphorus conditions
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丛枝菌根真菌在两种土壤磷条件下对寄主植物开花的影响不同
DOI：
10.1093/jpe/rtx038
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Chen, Xin
中科院分区：
文献类型：
--
作者：
Liu, Shijun;Guo, Hanling;Chen, Xin
目的研究表明丛枝菌根真菌(AMF)能极大地促进寄主植物的生长，但AMF对寄主植物开花物候的影响尚不清楚。通过盆栽试验，研究了在低磷和高磷条件下，丛枝菌根肥能否改变寄主紫花苜蓿的生活史和开花物候性状。方法在浙江大学中国温室内(东经120度19‘度，北纬30度26’度)，采用完全随机设计，设丛枝菌肥处理和土壤磷水平两因素。6种丛枝菌根真菌(Acaulospora scrobrulata，AS；Gigaspora margarita，Gma；Funneliformis geosporum，Fg；IntraradusRhizphagus inaradices，Ri；Funneliformis Mosseae，FMO和Glomus tortuosum，GT.)设计了两个土壤磷水平(24.0和5.7 mg kg(-1)Olsen可溶性磷)。这六种丛枝菌根真菌分别接种或混合接种(Mix)，并包括非丛枝菌根真菌对照(NAMF)。当植物开始开花时，每天都会记录每盆花的数量。在果实成熟期间，每天也记录成熟果实的数量。4个月后，测定寄主植物的生物量、生物量P含量和AMF定殖率。在低磷水平下，初花时间与根部定殖率和生物量P呈负相关，只有AMF种为AS、FG、Ri或Mix的寄主植物才能在播种后112d内完成生活史。AMF菌种FG、GT或AS处理在播种后112d内有两个开花高峰，而其他真菌处理只有一个开花高峰。FG树种的累积成花数和生物量P含量最高。寄主生物量分配因丛枝菌根真菌种类不同而有显著差异。在两种土壤磷水平下，寄主植物倾向于将更多的生物量分配给果实，而不是其他处理。这些结果表明，AMF对寄主开花物候和生物量分配的影响因AMF种类和土壤磷水平的不同而不同。
AimsStudies have showed that arbuscular mycorrhizal fungi (AMF) can greatly promote the growth of host plants, but how AMF affect flowering phenology of host plants is not well known. Here, we conducted a pot experiment to test whether life cycle and flowering phenology traits of host plant Medicago truncatula Gaertn can be altered by AMF under low and high soil phosphorus (P) levels.MethodsThe experiment was conducted in a greenhouse at Zhejiang University in China (120 degrees 19'E, 30 degrees 26'N) and had a completely randomized design with two factors: AMF treatments and soil P levels. Six AMF species (Acaulospora scrobiculata, As; Gigaspora margarita, Gma; Funneliformis geosporum, Fg; Rhizophagus intraradices, Ri; Funneliformis mosseae, Fmo and Glomus tortuosum, Gt.) were used, and two soil P levels (24.0 and 5.7 mg kg(-1) Olsen-soluble P) were designed. The six AMF species were separately inoculated or in a mixture (Mix), and a non-AMF control (NAMF) was included. When plants began to flower, the number of flowers in each pot was recorded daily. During fruit ripening, the number of mature fruits was also recorded daily. After similar to 4 months, the biomass, biomass P content and AMF colonization of host plant were measured. Correlation between root colonization and first flowering time, or P content and first flowering time was analyzed.Important FindingsUnder the low P level, first flowering time negatively correlated with root colonization and biomass P. Only host plants with AMF species As, Fg, Ri, or Mix were able to complete their life cycle within 112 days after sowing. And treatment with AMF species Fg, Gt, or As resulted in two periods of rapid flower production while other fungi treatments resulted in only one within 112 days after sowing. The cumulative number of flowers produced and biomass P content were highest with species Fg. Host biomass allocation significantly differed depending on the species of AMF. Under both soil P levels, the host plant tended to allocate more biomass to fruits in the Mix treatment than in the other treatments. These results indicated that the effects of AMF on host flowering phenology and biomass allocation differed depending on AMF species and soil P levels.