Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1
Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1
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DOI：
10.3168/jds.s0022-0302(03)73862-4
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发表时间：
2003-08-01
影响因子：
3.5
通讯作者：
Pulina, G
中科院分区：
文献类型：
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作者：
Battacone, G;Nudda, A;Pulina, G
进行了两项实验来研究牛奶中黄曲霉毒素 M1 (AFM1) 的含量对饲喂黄曲霉毒素 B1 (AFB1) 的反应。在实验 1 中，四只泌乳早期的奶羊接受单剂量的纯 AFB1（2 毫克）。在接下来的 5 天内收集各个牛奶样品以测量 AFM1 浓度。牛奶中 AFM1 的平均排泄量呈指数下降模式，在 24 小时和 48 小时有两个中间峰值。给药后 96 小时，牛奶中未检测到 AFM1。牛奶中 AFB1 向 AFM1 的平均转移率为 0.032%，个体差异较高（SD = 0.017%）。实验 2 将 16 只哺乳中期母羊分为 4 组，每天接受不同剂量的 AFB1（对照组分别为 0、32、64 和 128 杯，T1、T2 和 T3 组分别为 0、32、64 和 128 杯），持续 14 d。将纯AFB1分为每日两次剂量给予每只动物。首次施用 AFB1 后 12、24、36、48、72、96、144、216 和 312 小时、中毒期间以及停药后 7 天内每 24 小时收集一次乳样品。施用AFB1后12小时，在治疗组的所有动物的乳汁中检测到AFM1。在所有治疗组中，开始给药后 12 小时至 144 小时，牛奶 AFM1 浓度增加。然后下降，在首次给药后 216 和 312 小时达到稳定浓度。最后一次施用 AFB1 后 3 d，牛奶中未检测到 AFM1。稳态条件下测得的牛奶 AFM1 浓度显着受到 AFB1 剂量的影响（T1、T2 和 T3 组分别为 0.031、0.095 和 0.166），AFB1 剂量与牛奶 AFM1 浓度之间呈线性关系（R-2 = 77.2%）。残留（AFM1/AFB1 比值）未受治疗的显着影响，其平均值为 0.112%（SE = 0.011）。残留量低于奶牛和山羊报道的残留量，表明绵羊降解 AFB1 的能力更好。
Two experiments were conducted to study the amount of aflatoxin M1 (AFM1) in milk in response to feeding aflatoxin B1 (AFB1). In experiment 1, four dairy ewes in early lactation received a single dose of pure AFB1 (2 mg). Individual milk samples were collected during the following 5 d to measure AFM1 concentration. The average excretion of AFM1 in milk followed an exponential decreasing pattern, with two intermediate peaks at 24 and 48 h. No AFM1 was detected in milk at 96 h after dosing. The mean rate of transfer of AFB1 into AFM1 in milk was 0.032%, with a high individual variability (SD = 0.017%).In experiment 2, 16 dairy ewes in midlactation were divided into four groups that received different daily doses of AFB1 ( 0, 32, 64, and 128 mug for control and groups T1, T2, and T3, respectively) for 14 d. Pure AFB1 was administered to each animal divided in two daily doses. Individual milk samples were collected at 12, 24, 36, 48, 72, 96, 144, 216, and 312 h after the first AFB1 administration, during the intoxication period, and every 24 h for 7 d after the withdrawal of AFB1. AFM1 was detected in the milk of all animals of the treated groups at 12 h after the administration of AFB1. In all treated groups, milk AFM1 concentration increased from 12 to 144 h after the beginning of administration. It then decreased, reaching a stable concentration at 216 and 312 h after the first administration. No AFM1 was detected in milk 3 d after the last administration of AFB1. Milk AFM1 concentration measured at steady-state condition was significantly affected by the AFB1 dose (0.031, 0.095, and 0.166 in T1, T2, and T3 groups, respectively), with a linear relationship between AFB1 dose and milk AFM1 concentration (R-2 = 77.2%). The carryover (AFM1/AFB1 ratio) was not significantly affected by treatment, and its mean value was 0.112% ( SE = 0.011). The carryover was lower than that reported for dairy cattle and goats, suggesting a better ability of sheep to degrade AFB1.