Charge Sensitive Optical Detection for Measurement of Small-Molecule Binding Kinetics.
Charge Sensitive Optical Detection for Measurement of Small-Molecule Binding Kinetics.
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用于测量小分子结合动力学的电荷敏感光学检测。
DOI：
10.1007/978-1-0716-1803-5_17
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tao,Nongjian
中科院分区：
文献类型：
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作者：
Wang,Shaopeng;Ma,Guangzhong;Liang,Runli;Tao,Nongjian
电荷敏感光学检测（CSOD）技术是一种无标记的实时测量分子相互作用的方法。传统的无标签光学检测技术主要测量分子的质量，对小分子的灵敏度较低。相比之下，CSOD检测分子的电荷，其信号不会随着分子的大小而减弱，因此能够研究小分子。此外，CSOD与标准微孔板平台兼容，适用于候选药物的高通量筛选。在CSOD中，将带有探针分子的光纤浸入微孔板中，在微孔板上施加交替垂直电场，使光纤由于表面电荷的存在而进入振荡状态。目标分子的结合改变了光纤的电荷，从而改变了振荡光纤的振幅和相位，这是通过跟踪光纤尖端的光学图像精确测量的。
Charge sensitive optical detection (CSOD) technique is a label-free method for real-time measurement of molecular interactions. Traditional label-free optical detection techniques mostly measure the mass of a molecule, and they are less sensitive to small molecules. In contrast, CSOD detects the charge of a molecule, where the signal does not diminish with the size of the molecule, thus capable for studying small molecules. In addition, CSOD is compatible with the standard microplate platform, making it suitable for high-throughput screening of drug candidates. In CSOD, an optical fiber functionalized with the probe molecule is dipped into a well of a microplate where an alternate perpendicular electrical field is applied to the fiber, which drives the fiber into oscillation because of the presence of surface charge on the fiber. The binding of the target molecules changes the charge of the fiber, and thus the amplitude and phase of the oscillating fiber, which are precisely measured through tracking of the optical images of the fiber tip.