THE CULTURE OF CHICK-EMBRYO CHONDROCYTES AND THE CONTROL OF THEIR DIFFERENTIATED FUNCTIONS INVITRO .1. CHARACTERIZATION OF THE CHONDROCYTE-SPECIFIC PHENOTYPES
THE CULTURE OF CHICK-EMBRYO CHONDROCYTES AND THE CONTROL OF THEIR DIFFERENTIATED FUNCTIONS INVITRO .1. CHARACTERIZATION OF THE CHONDROCYTE-SPECIFIC PHENOTYPES
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DOI：
10.1016/0014-4827(82)90423-2
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
CANCEDDA, R
中科院分区：
文献类型：
--
作者：
CAPASSO, O;GIONTI, E;CANCEDDA, R
将鸡胚软骨细胞维持培养>2个月，在无酸的情况下传代漂浮细胞。在整个培养期间，一些细胞附着在培养皿上，呈现上皮样形态，随后产生新的漂浮细胞。两种细胞群的相互转化在原代中最高，并随着培养物老化而降低。软骨细胞在体外合成pro-α1(II)胶原蛋白和硫酸化蛋白聚糖；与飞蚊症相比，上皮样细胞分泌相对大量的纤连蛋白。当将抗坏血酸添加到培养基中时，所有细胞都附着并保持其圆形形状；在这种情况下，pro-alpha.1 (II)胶原蛋白成熟并且在细胞外可检测到胶原纤维。还鉴定了培养中的软骨细胞合成的其他特定蛋白质。其中一种是 64 K 胶原酶敏感蛋白，与 II 型胶原蛋白无关，可能代表一种新的胶原蛋白类型。
Chick embryo chondrocyte were maintained in culture for > 2 mo., passaging the floating cells in the absence acid. Throughout the culture period, some of the cells attached to the dish, assuming an epithelial-like morphology and subsequently giving rise to new floating cells. The interconversion of the 2 cell populations was highest in primaries and decreased with culture aging. Cartilage cells synthesized pro-.alpha.1 (II) collagen and sulfated proteoglycans in vitro; compared to floaters, the epithelial-like cells secreted relatively large amounts of fibronectin. When ascorbic acid was added to the medium, all cells attached, maintaining their rounded shape; in this condition the pro-.alpha.1 (II) collagen was matured and collagen fibers were detectable outside the cells. Other specific proteins synthesized by the chondrocytes in culture were also identified. One of these, a 64 K [kilodalton] collagenase-sensitive protein, was not related to the type II collagen and may represent a new collagen type.