Osthole inhibits histamine-dependent itch via modulating TRPV1 activity.
Osthole inhibits histamine-dependent itch via modulating TRPV1 activity.
复制标题
蛇床子素通过调节 TRPV1 活性抑制组胺依赖性瘙痒
DOI：
10.1038/srep25657
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发表时间：
2016-05-10
影响因子：
4.6
通讯作者：
Tang ZX
中科院分区：
文献类型：
--
作者：
Yang NN;Shi H;Yu G;Wang CM;Zhu C;Yang Y;Yuan XL;Tang M;Wang ZL;Gegen T;He Q;Tang K;Lan L;Wu GY;Tang ZX
蛇床子素是从蛇床子Cnidium monnieri（L.）蛇床子素是一种具有抗炎作用的中草药，但其抗炎作用机制尚不清楚。本研究通过行为学测试、钙离子成像和电生理实验，探讨了蛇床子素抗组胺依赖性瘙痒的分子机制。首先，蛇床子素明显缓解组胺、HTMT和VUF 8430诱导的小鼠抓挠行为。第二，在培养的背根神经节（DRG）神经元上，蛇床子素对组胺有剂量依赖性的抑制作用。在相同的神经元上，蛇床子素也降低辣椒素和组胺的反应。在进一步的实验中，辣椒素诱导的内向电流被蛇床子素抑制。这些结果表明，蛇床子素通过调节TRPV1活性抑制组胺依赖性瘙痒。本研究将有助于了解蛇床子素如何发挥抗瘙痒作用，并提示蛇床子素可能是一种有用的治疗组胺依赖性瘙痒的药物。
Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca2+ imaging, and electrophysiological experiments. First, osthole clearly remitted the scratching behaviors of mice induced with histamine, HTMT, and VUF8430. Second, in cultured dorsal root ganglion (DRG) neurons, osthole showed a dose-dependent inhibitory effect to histamine. On the same neurons, osthole also decreased the response to capsaicin and histamine. In further tests, the capsaicin-induced inward currents were inhibited by osthole. These results revealed that osthole inhibited histamine-dependent itch by modulating TRPV1 activity. This study will be helpful in understanding how osthole exerts anti-pruritus effects and suggests that osthole may be a useful treatment medicine for histamine-dependent itch.