Ca2+-dependent interaction of BAPTA with phospholipids
Ca2+-dependent interaction of BAPTA with phospholipids
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DOI：
10.1016/j.febslet.2004.08.058
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发表时间：
2004-10-08
期刊：
影响因子：
3.5
通讯作者：
Charnet, P
中科院分区：
文献类型：
--
作者：
Rousset, M;Cens, T;Charnet, P
从不同 Ca2+ 螯合剂对 G 蛋白诱导的 Ca(v)2.1 Ca 通道抑制的比较研究开始，我们证明 BAPTA 和 DM-硝基酚能够以 Ca2+ 和脂质依赖性方式与磷脂单层相互作用。临界插入压力和对带电脂质的敏感性表明，脂质膜中的插入可能发生在生物膜中，就像爪蟾卵母细胞上发现的那样。 EGTA 和 EDTA 没有发现这种新特性，并且可能与 BAPTA 相关分子在质膜附近螯合 Ca2+ 离子的不寻常能力有关，大多数由电压门控 Ca2+ 电流触发的 Ca2+ 依赖性信号传导发生在质膜附近。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Starting from a comparative study of different Ca2+ chelators on the G-protein-induced inhibition of the Ca(v)2.1 Ca channels, we demonstrate that BAPTA and DM-nitrophen are able to interact, in a Ca2+- and lipid-dependent manner, with phospholipid monolayers. Critical insertion pressure and sensitivity to charged lipids indicated that insertion in the lipid film may occur in biological membranes as those found on Xenopus oocytes. This novel property is not found for EGTA and EDTA and may participate to the unusual ability of BAPTA-related molecules to chelate Ca2+ ions in the very close vicinity of the plasma membrane, where most of the Ca2+-dependent signalling triggered by voltage-gated Ca2+ currents occurs. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.