DNA microarrays: a powerful genomic tool for biomedical and clinical research
DNA microarrays: a powerful genomic tool for biomedical and clinical research
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DOI：
10.2119/2006-00107.trevino
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发表时间：
2007-09-01
影响因子：
5.7
通讯作者：
Barrera-Saldana, Hugo A.
中科院分区：
文献类型：
--
作者：
Trevino, Victor;Falciani, Francesco;Barrera-Saldana, Hugo A.
人类基因组计划的诸多好处之一是新的和强大的工具，如被称为微阵列的全基因组杂交设备，最初设计用于测量基因转录水平，微阵列技术现在用于比较个体及其组织和细胞之间的其他基因组特征。结果提供了关于疾病亚类、疾病预后和治疗结果的宝贵信息，同样，它们揭示了基因组成、调节机制和微妙变化的差异，使我们更接近个性化医疗的时代。为了了解这个强大的工具，它的多功能性，以及它如何戏剧性地改变生物医学和临床研究的分子方法，这篇综述描述了这项技术，它的应用，一个典型的微阵列协议的教学一步一步的回顾，以及一个真实的实验。最后，它呼吁医学界注意整合多学科团队的重要性，以利用这项技术及其不断扩大的应用，在幻灯片中揭示了我们的基因遗传和命运。
Among the many benefits of the Human Genome Project are new and powerful tools such as the genome-wide hybridization devices referred to as microarrays, Initially designed to measure gene transcriptional levels, microarray technologies are now used for comparing other genome features among individuals and their tissues and cells. Results provide valuable information on disease subcategories, disease prognosis, and treatment outcome, Likewise, they reveal differences in genetic makeup, regulatory mechanisms, and subtle variations and move us closer to the era of personalized medicine. To understand this powerful tool, its versatility, and how dramatically it is changing the molecular approach to biomedical and clinical research, this review describes the technology, its applications, a didactic step-by-step review of a typical microarray protocol, and a real experiment. Finally, it calls the attention of the medical community to the importance of integrating multidisciplinary teams to take advantage of this technology and its expanding applications that, in a slide, reveals our genetic inheritance and destiny.