D1 AND D2 DOPAMINE RECEPTOR MESSENGER-RNA IN RAT-BRAIN
D1 AND D2 DOPAMINE RECEPTOR MESSENGER-RNA IN RAT-BRAIN
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DOI：
10.1073/pnas.88.5.1859
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
BRANN, MR
中科院分区：
文献类型：
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作者：
WEINER, DM;LEVEY, AI;BRANN, MR
生理学和药理学标准已将多巴胺受体分为D1和D2亚型，编码这些亚型的基因最近已被克隆。 根据所克隆的受体序列，我们制备了寡核苷酸探针，通过原位杂交组织化学方法，定位了相应mRNA在大鼠脑内的细胞表达。 这些mRNA显示出很大程度上重叠但不同的表达模式。 两种mRNA的最高表达水平在尾壳核、丘脑核和嗅结节中观察到。 在尾壳核内，47 +/- 6%和46 +/- 5%的中型神经元（10-15 μ m）分别表达D1和D2 mRNA，而在较大的神经元（> 20 μ m）中仅观察到D2 mRNA。 D1和D2 mRNA在大多数皮质区域中表达，在前额叶和内嗅皮质中表达水平最高。 在新皮质内，D1 mRNA主要在第6层中观察到，D2 mRNA在第4-5层中观察到。 在杏仁核内，D1 mRNA见于闰核，D2 mRNA见于中央核。 在下丘脑，D1 mRNA中观察到的视交叉上核和D2 mRNA中的许多多巴胺能细胞群。 在隔、苍白球、上级和下丘、乳头体和黑质内仅检测到D2 mRNA。 这些数据提供了深入了解作用于D1或D2受体的药物的不同作用的神经解剖学基础。
Physiological and pharmacological criteria have divided dopamine receptors into D1 and D2 subtypes, and genes encoding these subtypes have recently been cloned. Based on the sequences of the cloned receptors, we prepared oligodeoxynucleotide probes to map the cellular expression of the corresponding mRNAs in rat brain by in situ hybridization histochemistry. These mRNAs showed largely overlapping yet distinct patterns of expression. The highest levels of expression for both mRNAs were observed in the caudate-putamen, nucleus accumbens, and olfactory tubercle. Within the caudate-putamen, 47 +/- 6% and 46 +/- 5% of the medium-sized neurons (10-15-mu-m) expressed the D1 and D2 mRNAs, respectively, and only the D2 mRNA was observed in the larger neurons (> 20-mu-m). The D1 and D2 mRNAs were expressed in most cortical regions, with the highest levels in the prefrontal and entorhinal cortices. Within neocortex, D1 mRNA was observed primarily in layer 6 and D2 mRNA in layers 4-5. Within the amygdala, D1 mRNA was observed in the intercalated nuclei, and D2 mRNA in the central nucleus. Within the hypothalamus, D1 mRNA was observed in the suprachiasmatic nucleus and D2 mRNA in many of the dopaminergic cell groups. Within the septum, globus pallidus, superior and inferior colliculi, mammillary bodies, and substantia nigra only D2 mRNA was detected. These data provide insight into the neuroanatomical basis of the differential effects of drugs that act on D1 or D2 receptors.