Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus
Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus
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耐甲氧西林金黄色葡萄球菌FtsA的表达、纯化、结晶及初步晶体学研究
DOI：
10.1107/s1744309113017727
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and H. Matsumura
中科院分区：
文献类型：
--
作者：
J. Fujita;Y. Miyazaki;M. Hirose;C. Nagao;E. Mizohata;Y. Matsumoto;K. Mizuguchi;T. Inoue;and H. Matsumura
对耐甲氧西林金黄色葡萄球菌（MRSA）的FtsA进行了克隆、过表达和纯化。用坐滴蒸汽扩散技术结晶蛋白质。用PEG 3350作为沉淀剂，在293 K下培养β-γ-氨基腺苷5 ' -磷酸（AMPPNP，一种不可水解的ATP类似物）共晶。在100 K下采集x射线衍射数据，分辨率为2.3 Å。该晶体属于单斜空间群P21，晶胞参数a = 75.31, b = 102.78, c = 105.90 Å， β = 96.54°。计算出的马修斯系数表明，不对称单元包含三个或四个单体。
FtsA from methicillin-resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting-drop vapour-diffusion technique. A cocrystal with β-γ-imidoadenosine 5′-phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as a precipitant at 293 K. X-ray diffraction data were collected to a resolution of 2.3 Å at 100 K. The crystal belonged to the monoclinic space group P21, with unit-cell parameters a = 75.31, b = 102.78, c = 105.90 Å, β = 96.54°. The calculated Matthews coefficient suggested that the asymmetric unit contained three or four monomers.