Differentiation and quantification of Theileria sergenti piroplasm types using type-specific monoclonal antibodies
Differentiation and quantification of Theileria sergenti piroplasm types using type-specific monoclonal antibodies
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DOI：
10.1292/jvms.60.665
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发表时间：
1998-06-01
影响因子：
1.2
通讯作者：
Onuma, M
中科院分区：
文献类型：
--
作者：
Iwasaki, T;Kakuda, T;Onuma, M
对瑟氏泰勒氏菌主要胞浆表面蛋白(MPSP)两种代表性等位基因产物的抗原性进行了研究。免疫印迹实验显示，感染Ikeda和Chitose两种寄生虫的牛血清与同源重组抗原反应强，而与异源重组抗原反应弱。针对MPSP寄生虫的两种等位基因产物产生的单抗只与免疫抗原反应。这些结果表明，两个等位基因产物之间的交叉反应性很低，尽管它们的氨基酸序列具有较高的同源性。通过间接免疫荧光法对寄生虫型特异性MoAbs的红细胞涂片进行双重染色，结果表明，该套MoAbs可用于混合人群中各种寄生虫的定量和鉴别检测。
Antigenic properties of two representative allelic products of the major piroplasm surface protein (MPSP) of Theileria sergenti were studied. Sera from cattle infected with either of Ikeda and Chitose types of the parasite reacted strongly with homologous but weakly with heterologous recombinant antigens in immunoblotting. Monoclonal antibodies (MoAbs) produced against the both allelic products of MPSP parasites reacted only to the immunizing antigen. These results suggested that crossreactivity between two allelic products is very low inspite of relatively high homology in their amino acid sequences. Double staining of parasitized erythrocyte smear using type-specific MoAbs by an indirect immunofluorescent assay revealed that the set of MoAbs was useful for quantitative and differential detection of each type of parasite in mixed population.