Techniques to Quantify cGMP Dysregulation as a Common Pathway Associated with Photoreceptor Cell Death in Retinitis Pigmentosa.
Techniques to Quantify cGMP Dysregulation as a Common Pathway Associated with Photoreceptor Cell Death in Retinitis Pigmentosa.
复制标题
量化 cGMP 失调作为与视网膜色素变性感光细胞死亡相关的常见途径的技术。
DOI：
10.1007/978-3-030-27378-1_67
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发表时间：
2019
影响因子：
--
通讯作者：
Titus,Hope
中科院分区：
文献类型：
--
作者：
Yang,Paul;Lockard,RachelA;Titus,Hope
色素性视网膜炎（RP）的神经保护疗法的有针对性的发展取决于对光感受器细胞死亡机制的更好理解。核苷酸代谢物相关的光感受器细胞死亡是一个新兴的研究领域，在多种RP模型中很重要，但确切的病理生理机制仍有待阐明。RP中光感受器细胞死亡的一个常见途径是cGMP失调，其可能与高达30%的RP患者相关。优化检测和定量视网膜中核苷酸代谢物的工具对于扩大这一研究领域至关重要。免疫组织化学有助于以细胞特异性的方式定位异常高水平的cGMP，而酶联免疫吸附测定和液相色谱-质谱法是定量的，更敏感。这些技术可以为更复杂的实验奠定基础，以阐明光感受器细胞死亡的上游事件，这将有望导致RP患者新疗法的发展。
The targeted development of neuroprotective therapies for retinitis pigmentosa (RP) depends upon a better understanding of the mechanisms of photoreceptor cell death. Nucleotide metabolite-associated photoreceptor cell death is an emerging area of research that is important in multiple models of RP, yet the exact pathophysiology remains to be elucidated. One common pathway of photoreceptor cell death in RP is cGMP dysregulation, which is underscored by its potential to be relevant in up to 30% of patients with RP. Optimizing tools for detecting and quantifying nucleotide metabolites in the retina is vital to expanding this area of research. Immunohistochemistry is useful for localizing abnormally high levels of cGMP in a cell-specific manner, while enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry are quantitative and more sensitive. These techniques can form the basis for more sophisticated experiments to elucidate upstream events in photoreceptor cell death, which will hopefully lead to the development of novel therapies for patients with RP.