EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
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DOI：
10.1038/nmeth.3541
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发表时间：
2015-10
期刊：
影响因子：
48
通讯作者：
Fraser JS
中科院分区：
文献类型：
--
作者：
Barad BA;Echols N;Wang RY;Cheng Y;DiMaio F;Adams PD;Fraser JS
高分辨率电子冷冻显微镜(CRYO-EM)的进步伴随着独立评估地图质量和模型几何的验证指标的发展。EMRinger评估了在细化过程中原子模型与地图的精确拟合，并展示了辐射损伤如何改变带负电荷的氨基酸的散射。EMRinger将有助于监测在解决和模拟低温EM高分辨率特征方面的进展。
Advances in high resolution electron cryomicroscopy (cryo-EM) have been accompanied by the development of validation metrics to independently assess map quality and model geometry. EMRinger assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.