3 DISTINCT ANTIGENS ASSOCIATED WITH HUMAN LYMPHOCYTE-T-MEDIATED CYTOLYSIS - LFA-1, LFA-2, AND LFA-3
3 DISTINCT ANTIGENS ASSOCIATED WITH HUMAN LYMPHOCYTE-T-MEDIATED CYTOLYSIS - LFA-1, LFA-2, AND LFA-3
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DOI：
10.1073/pnas.79.23.7489
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
SPRINGER, TA
中科院分区：
文献类型：
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作者：
SANCHEZMADRID, F;KRENSKY, AM;SPRINGER, TA
制备抗HLA-DR溶细胞性T淋巴细胞（CTL）的单克隆抗体，并筛选其对CTL介导的杀伤的抑制作用。单克隆抗体与4种分子LFA-1、LFA-2、LFA-3和HLA-DR结合后，杀伤作用均受到抑制，提示这些分子参与了CTL与靶细胞的相互作用。用免疫沉淀、交联、十二烷基硫酸钠/聚丙烯酰胺凝胶电泳和免疫荧光流式细胞术对抗原进行了表征。LFA-1抗原含有α。和βMW为177，000和95，000的多肽链，其以α 1 β 1结构非共价结合。它存在于B和T淋巴细胞上，并标记在定量表达上不同的亚群。人LFA-1似乎是小鼠LFA-1的同源物。人LFA-2的分子量为49，000，其次要成分为36，000。它在CTL细胞系上表达，但在B细胞系上不表达，并且在外周血中优先在T淋巴细胞上表达。人LFA-3分子量为60，000，在B和T淋巴细胞上表达。
Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTL) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to 4 types of molecules, LFA-1, LFA-2, LFA-3 and HLA-DR inhibited killing, suggesting that these molecules partricipate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, sodium dodecyl sulfate/polyacrylamide gel electrophoresis and immunofluorescence flow cytometry. The LFA-1 antigen contains .alpha. and .beta. polypeptide chains of MW 177,000 and 95,000 that are noncovalently associated in an .alpha.1.beta.1 structure. It it present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homolog of mouse LFA-1. Human LFA-2 is of MW 49,000 with a minor component of MW 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of MW 60,000 and is expressed on both B and T lymphocytes.